Ezaki O
Division of Nutrition and Diseases, National Institute of Nutrition, Tokyo, Japan.
J Biol Chem. 1990 Jan 15;265(2):1124-8.
Selenate was found to have several insulin-like effects in rat adipocytes: stimulation of glucose transport activity by translocation of two types of glucose transporters from intracellular sites to the plasma membrane, stimulation of cAMP phosphodiesterase activity, and stimulation of ribosomal S6 protein phosphorylation. Furthermore, in intact cells addition of 1 mM selenate stimulated tyrosyl phosphorylation of 210-, 170-, 120-, 95-, 70-, and 60-kDa proteins but failed to stimulate insulin receptor kinase activity, suggesting that selenate stimulated other tyrosine kinase. In the presence of insulin, selenate enhances insulin receptor kinase activity and phosphorylations of insulin-stimulated tyrosyl phosphoproteins. These results may provide clues for the elucidation of the role of selenium in animals and the mechanism of insulin action.
通过将两种类型的葡萄糖转运蛋白从细胞内位点转运至质膜来刺激葡萄糖转运活性，刺激环磷酸腺苷磷酸二酯酶活性，以及刺激核糖体S6蛋白磷酸化。此外，在完整细胞中添加1 mM硒酸盐可刺激210 kDa、170 kDa、120 kDa、95 kDa、70 kDa和60 kDa蛋白的酪氨酰磷酸化，但未能刺激胰岛素受体激酶活性，这表明硒酸盐刺激了其他酪氨酸激酶。在胰岛素存在的情况下，硒酸盐可增强胰岛素受体激酶活性以及胰岛素刺激的酪氨酰磷蛋白的磷酸化。这些结果可能为阐明硒在动物中的作用及胰岛素作用机制提供线索。
