Satoh S
Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi.
Nihon Naibunpi Gakkai Zasshi. 1992 May 20;68(5):557-67. doi: 10.1507/endocrine1927.68.5_557.
Human amniotic fluid contains a very high concentration of PRL. And it is widely accepted that decidual cells are the origin of amniotic fluid PRL, and decidual cell PRL is transported into amniotic fluid across the fetal membranes. However, the decidual cells are, in their lower aspect, in contact with chorion cells. Because of this complex structural relationship, decidual cells are often called decidua-chorion cells, besides, chorion PRL reproduction remains to be elucidated. Consequently, we further examined tissue of chorion laeve which had been obtained at spontaneous term vaginal delivery. The results of our examinations are as follows; 1. Chorion PRL was determined with precipitation method of radioimmunoassay, and it was concluded that chorion tissue contains no small amounts of human PRL; 391 +/- 24 ng/g in tissue, whereas decidual PRL was 225 +/- 91 ng/g in tissue. 2. Using immunohistochemical procedures and PAP complex methods, immunized anti-ovine PRL serum revealed PRL reactivity around the numerous granules or globules in term chorion cells. These data suggest that the chorion cells are the site of reproducible PRL localization and synthesis and, comparable to decidual cells, are the possible source of human PRL in term fetal membrane.
人羊水含有非常高浓度的催乳素（PRL）。人们普遍认为蜕膜细胞是羊水PRL的来源，蜕膜细胞PRL通过胎膜转运到羊水中。然而，蜕膜细胞在其下部与绒毛膜细胞接触。由于这种复杂的结构关系，蜕膜细胞常被称为蜕膜 - 绒毛膜细胞，此外，绒毛膜PRL的产生仍有待阐明。因此，我们进一步检查了在足月自然阴道分娩时获得的平滑绒毛膜组织。我们的检查结果如下：1. 用放射免疫沉淀法测定绒毛膜PRL，得出绒毛膜组织含有相当数量的人PRL；组织中为391±24 ng/g，而蜕膜PRL在组织中为225±91 ng/g。2. 使用免疫组织化学程序和PAP复合物方法，免疫的抗羊PRL血清在足月绒毛膜细胞中的许多颗粒或小球周围显示出PRL反应性。这些数据表明绒毛膜细胞是PRL定位和合成的部位，并且与蜕膜细胞类似，是足月胎膜中人PRL的可能来源。
