Kim In-Yong, Yoo Mi-Kyong, Kim Bom-Chol, Kim Se-Kwon, Lee Hyun-Chul, Cho Chong-Su
School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, South Korea.
Int J Biol Macromol. 2006 Feb 28;38(1):51-8. doi: 10.1016/j.ijbiomac.2005.12.020. Epub 2006 Jan 26.
Through semi-interpenetration of polymer networks with poloxamer, mechanical properties of chitosan (CS) sponge were increased for wound dressing application. Synthesis of poloxamer macromer was confirmed by proton nuclear magnetic resonance (1H NMR) spectra. Possible interactions between CS and poloxamer in semi-interpenetrating polymer networks (SIPNs), and changes in crystalline structures of both polymers were evaluated by Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD), respectively. Swelling behavior, thermal analysis, mechanical properties, and morphology of SIPNs were studied by thermal gravimetric analysis, differential scanning calorimetry (DSC), compressive modulus measurement, and scanning electron microscopy (SEM), respectively. Preparation of poloxamer macromer, and intermolecular hydrogen bonding between CS and poloxamer were confirmed by NMR and FTIR, respectively. Melting temperature of poloxamer in SIPNs decreased due to prevention of crystallization by incorporation of CS. Formation of SIPNs with poloxamer and increasing poloxamer content in CS/poloxamer SIPNs increased mechanical strength of CS sponge compared with CS/poloxamer blend. Formation of SIPNs with poloxamer remarkably increased water content of CS due to hydrophilicity of CS and poloxamer. These results suggest CS/poloxamer sponges prepared by SIPNs method have good possibility for wound dressing application owing to rapid water adsorption, high mechanical strength, and interconnected cross-sectional morphology of SIPNs.
通过聚合物网络与泊洛沙姆的半互穿，壳聚糖（CS）海绵的机械性能得以提高，可用于伤口敷料。通过质子核磁共振（1H NMR）光谱证实了泊洛沙姆大分子单体的合成。分别通过傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）评估了半互穿聚合物网络（SIPNs）中CS与泊洛沙姆之间可能的相互作用以及两种聚合物晶体结构的变化。分别通过热重分析、差示扫描量热法（DSC）、压缩模量测量和扫描电子显微镜（SEM）研究了SIPNs的溶胀行为、热分析、机械性能和形态。分别通过NMR和FTIR证实了泊洛沙姆大分子单体的制备以及CS与泊洛沙姆之间的分子间氢键。由于CS的掺入阻止了结晶，SIPNs中泊洛沙姆的熔点降低。与CS/泊洛沙姆共混物相比，泊洛沙姆与CS形成SIPNs以及CS/泊洛沙姆SIPNs中泊洛沙姆含量的增加提高了CS海绵的机械强度。由于CS和泊洛沙姆的亲水性，泊洛沙姆与CS形成SIPNs显著增加了CS的含水量。这些结果表明，通过SIPNs方法制备的CS/泊洛沙姆海绵由于其快速的水吸附、高机械强度以及SIPNs相互连接的横截面形态，在伤口敷料应用方面具有很大的可能性。
