Kweon Haeyong, Yeo Joo-hong, Lee Kwang-gill, Lee Hyun Chul, Na Hee Sam, Won Young Ho, Cho Chong Su
Applied Sericulture and Apiculture Division, National Institute of Agricultural Science and Technology, Suwon 441100, Korea.
Biomed Mater. 2008 Sep;3(3):034115. doi: 10.1088/1748-6041/3/3/034115. Epub 2008 Aug 15.
Semi-interpenetrating polymer networks (SIPNs) composed of silk fibroin (SF) and poly(ethylene glycol) (PEG) were prepared by photopolymerization of a PEG macromer in the presence of SF to improve the mechanical properties of SF sponge as wound dressing. The morphological structure of the SF/PEG SIPNs was observed to be composed of an interconnected microporous surface and a cross-sectional area. SF/PEG SIPNs showed non-cytotoxicity evaluated by a cell proliferation method using L929 fibroblasts. Wound contraction treated with SF/PEG SIPNs sponges was faster than that of Vaseline gauze as a control. Histological observation confirmed that the deposition of collagen in the dermis was organized by covering the wound area with SF/PEG SIPNs. The above results indicated that SF/PEG SIPNs could be used as wound dressing.
通过在丝素蛋白（SF）存在下对聚乙二醇（PEG）大分子单体进行光聚合反应，制备了由丝素蛋白（SF）和聚乙二醇（PEG）组成的半互穿聚合物网络（SIPN），以改善作为伤口敷料的SF海绵的机械性能。观察到SF/PEG SIPN的形态结构由相互连接的微孔表面和横截面积组成。通过使用L929成纤维细胞的细胞增殖方法评估，SF/PEG SIPN显示无细胞毒性。用SF/PEG SIPN海绵处理的伤口收缩比作为对照的凡士林纱布更快。组织学观察证实，通过用SF/PEG SIPN覆盖伤口区域，真皮中胶原蛋白的沉积得到了组织化。上述结果表明，SF/PEG SIPN可作为伤口敷料使用。
