Bugaj Andrzej, Juzeniene Asta, Juzenas Petras, Iani Vladimir, Ma Li Wei, Moan Johan
Department of Clinical Pharmacy and Biopharmacy, Karol Marcinkowski University of Medical Sciences, ul. Marii Magdaleny 14, 61861 Poznań, Poland.
J Photochem Photobiol B. 2006 May 1;83(2):94-7. doi: 10.1016/j.jphotobiol.2005.12.003. Epub 2006 Jan 26.
The influence of skin permeation enhancers, such as dimethyl sulphoxide (DMSO) and 1-[2-(decylthio)ethyl]azacyclopentan-2-one (HPE-101), Labrafac CC, Labrafil, Labrasol and Transcutol in a concentration of 10% (wt./wt.) on the formation of porphyrins in normal mouse skin from topical application of creams with methyl 5-aminolevulinate (MAL) was studied. The concentration of porphyrins in the mouse skin was determined by direct fluorescence measurements. The results show that studied permeation enhancers increase the formation of porphyrins, and therefore also the skin penetration 2% MAL whereas for 10% and 20% (wt./wt.) MAL concentrations only DMSO, HPE-101 and Labrafac CC increased the porphyrin formation. At all studied MAL concentrations DMSO gave the largest enhancing effect, similarly to that of HPE-101. This suggests that in 2-20% MAL creams HPE-101 may be substituted by Labrafac CC to reduce skin irritation induced by HPE-101 without impairing the porphyrin formation.
研究了浓度为10%（重量/重量）的皮肤渗透促进剂，如二甲基亚砜（DMSO）、1-[2-（癸硫基）乙基]氮杂环戊烷-2-酮（HPE-101）、Labrafac CC、Labrafil、Labrasol和Transcutol，对局部应用含5-氨基乙酰丙酸甲酯（MAL）的乳膏后正常小鼠皮肤中卟啉形成的影响。通过直接荧光测量法测定小鼠皮肤中卟啉的浓度。结果表明，所研究的渗透促进剂会增加卟啉的形成，从而也会增加2% MAL的皮肤渗透；而对于10%和20%（重量/重量）的MAL浓度，只有DMSO、HPE-101和Labrafac CC会增加卟啉的形成。在所有研究的MAL浓度下，DMSO的增强作用最大，与HPE-101类似。这表明在2%-20% MAL乳膏中，HPE-101可用Labrafac CC替代，以减少HPE-101引起的皮肤刺激，同时不影响卟啉的形成。
