Oztekin Esma, Tiftik Ali Muhtar, Baltaci Abdulkerim Kasim, Mogulkoc Rasim
Department of Biochemistry, Meram Medical School, Selcuk University, Turkey.
Cell Biochem Funct. 2007 Jul-Aug;25(4):401-5. doi: 10.1002/cbf.1313.
The present study aimed to determine the effect of estradiol-progesterone supplementation and pinealectomy on lipid peroxidation of liver tissue in ovariectomized rats. The study was carried out on 36 adult Sprague-Dawley female rats, which weighed 200-250 g. The rats were divided into 6 groups: Group 1: Sham Ovariectomy (Sham-Ovx), Group 2: Ovariectomy (Ovx), Group 3: Ovx + Estradiol-Progesterone supplementation (Ovx + H), Group 4: Sham Pinealectomy and Ovx (Sham Pnx -Ovx), Group 5: Ovx -Pnx, Group 6: Ovx -Pnx + H. Malondialdehyde (MDA), reduced form of glutathione (GSH) and glutathione peroxidase (GSH-Px) levels were determined in liver tissue of rats. The highest MDA levels and the lowest GSH-Px levels were determined in the ovariectomized-pinealectomized group, whereas the lowest MDA was in the Sham-Ovx group, and the highest GSH-Px levels were found in the Sham-Ovx and Ovx + Hormone supplemented group. Furthermore, the highest GSH levels were in group 1 and lowest levels were in group 5. The findings of this study demonstrate that ovariectomy led to lipid peroxidation in liver tissues of rats. Pinealectomy in addition to ovariectomy, increases lipid peroxidation, but, estradiol and progesterone supplementations to the ovariectomized-pinealectomized rats protect against lipid peroxidation to a significant extent.
本研究旨在确定补充雌二醇 - 孕酮及松果体切除对去卵巢大鼠肝组织脂质过氧化的影响。本研究以36只体重200 - 250克的成年Sprague - Dawley雌性大鼠为实验对象。大鼠被分为6组：第1组：假去卵巢组（假去势组）；第2组：去卵巢组（去势组）；第3组：去卵巢 + 雌二醇 - 孕酮补充组（去势 + H组）；第4组：假松果体切除及去卵巢组（假松果体切除 - 去势组）；第5组：去卵巢 - 松果体切除组；第6组：去卵巢 - 松果体切除 + H组。测定大鼠肝组织中丙二醛（MDA）、还原型谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GSH - Px）水平。去卵巢 - 松果体切除组MDA水平最高，GSH - Px水平最低；假去卵巢组MDA水平最低，假去卵巢组和去卵巢 + 激素补充组GSH - Px水平最高。此外，第1组GSH水平最高，第5组最低。本研究结果表明，去卵巢导致大鼠肝组织脂质过氧化。去卵巢后再行松果体切除会增加脂质过氧化，但对去卵巢 - 松果体切除大鼠补充雌二醇和孕酮可在很大程度上防止脂质过氧化。
