Yu Byung Jo, Sung Bong Hyun, Lee Ju Young, Son Sung Hwa, Kim Mi Sun, Kim Sun Chang
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea.
FEMS Microbiol Lett. 2006 Jan;254(2):245-50. doi: 10.1111/j.1574-6968.2005.00026.x.
sucAB and sucCD of Escherichia coli encode enzymes that generate succinyl-CoA from 2-oxoglutarate and succinate, respectively. Their mutual essentiality was studied. sucAB and sucCD could be deleted individually, but not simultaneously. The mutual essentiality of sucAB and sucCD was further confirmed by the conditional expression of sucABCD, sucAB, and sucCD under the control of a P(BAD) in E. coli MG1655, E. coli MG1655 (DeltasucCD), and E. coli MG1655 (DeltasucAB), respectively. These strains grew well in Luria-Bertani medium containing 0.1% arabinose, but not in the absence of arabinose unless the medium was supplemented with succinyl-CoA. Our results indicate that either sucAB or sucCD is enough to produce succinyl-CoA that is essential for cell viability.
大肠杆菌的sucAB和sucCD分别编码从2-氧代戊二酸和琥珀酸生成琥珀酰辅酶A的酶。对它们的相互必要性进行了研究。sucAB和sucCD可以单独缺失，但不能同时缺失。分别在大肠杆菌MG1655、大肠杆菌MG1655（DeltasucCD）和大肠杆菌MG1655（DeltasucAB）中，通过在P（BAD）控制下对sucABCD、sucAB和sucCD进行条件表达，进一步证实了sucAB和sucCD的相互必要性。这些菌株在含有0.1%阿拉伯糖的Luria-Bertani培养基中生长良好，但在没有阿拉伯糖的情况下生长不佳，除非培养基中添加了琥珀酰辅酶A。我们的结果表明，sucAB或sucCD中的任何一个都足以产生对细胞活力至关重要的琥珀酰辅酶A。
