Pedersen-Bjergaard Stig, Rasmussen Knut Einar
School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.
J Chromatogr A. 2006 Mar 24;1109(2):183-90. doi: 10.1016/j.chroma.2006.01.025. Epub 2006 Jan 30.
Basic drug substances were transported across a thin artificial organic liquid membrane by the application of 300 V d.c. From a 300 microl aqueous donor compartment (containing 10 mM HCl), the drugs migrated through a 200 microm artificial liquid membrane of 2-nitrophenyl octyl ether immobilized in the pores of a polypropylene hollow fiber, and into a 30 microl aqueous acceptor solution of 10 mM HCl inside the lumen of the hollow fiber. The transport was forced by an electrical potential difference sustained over the liquid membrane, resulting in electrokinetic migration of drug substances from the donor compartment to the acceptor solution. Within 5 min of operation at 300 V, pethidine, nortriptyline, methadone, haloperidol, and loperamide were extracted with recoveries in the range 70-79%, which corresponded to enrichments in the range 7.0-7.9. The chemical composition of the organic liquid membrane strongly affected the permeability, and may serve as an efficient tool for controlling the transport selectivity. Water samples, human plasma, and human urine were successfully processed, and in light of the present report, electrokinetic migration across thin artificial liquid membranes may be an interesting tool for future isolation within chemical analysis.
通过施加300 V直流电，使碱性药物物质穿过一层薄的人工有机液膜。药物从300微升含10 mM盐酸的水相供体隔室中迁移出来，穿过固定在聚丙烯中空纤维孔中的200微米厚的2-硝基苯基辛基醚人工液膜，进入中空纤维内腔中30微升含10 mM盐酸的水相受体溶液。液膜上维持的电势差促使了这种迁移，导致药物物质从供体隔室向受体溶液进行电动迁移。在300 V下操作5分钟内，哌替啶、去甲替林、美沙酮、氟哌啶醇和洛哌丁胺的萃取回收率在70 - 79%范围内，这相当于富集倍数在7.0 - 7.9范围内。有机液膜的化学成分强烈影响渗透性，并且可作为控制传输选择性的有效工具。水样、人血浆和人尿液均成功处理，根据本报告，穿过薄人工液膜的电动迁移可能是未来化学分析中用于分离的一个有趣工具。
