Department of Chemistry, Faculty of Basic Sciences, Tarbiat Modares University, Tehran 14115-175, Iran.
Biosensors (Basel). 2023 Jan 14;13(1):139. doi: 10.3390/bios13010139.
In the present study, on-chip electromembrane surrounded solid phase microextraction (EM-SPME) was employed in the determination of tricyclic antidepressants (TCAs), including amitriptyline, nortriptyline, imipramine, desipramine, maprotiline, and sertraline, from various biological fluids. In this regard, poly(3,4-ethylenedioxythiophene)-graphene oxide (PEDOT-GO) was electrodeposited on an SPME fiber as a conductive coating, then the fiber played the acceptor-electrode role during the extraction. Thus, the immigration of the analytes under the influence of an electric field and their absorption onto the fiber coating were accomplished simultaneously. Under the optimized conditions, the limits of detection for the target analytes were acquired in the range of 0.005-0.025 µg L using gas chromatography-mass spectrometry. The linearity of the method was 0.010-500 µg L for the imipramine and sertraline, 0.025-500 µg L for the amitriptyline, nortriptyline, and desipramine, and 1.000-250 µg L for the maprotiline (R ≥ 0.9984). Moreover, this method provided suitable precision and fiber-to-fiber reproducibility, with RSDs ≤ 8.4%. The applicability of the proposed setup was eventually investigated for extraction of the drugs from human bone marrow aspirate, urine, plasma, and well water samples, in which satisfactory relative recoveries, from 93-105%, were obtained.
在本研究中，采用芯片上电膜包围固相微萃取（EM-SPME）法从各种生物流体中测定三环抗抑郁药（TCAs），包括阿米替林、去甲替林、丙咪嗪、去甲丙咪嗪、马普替林和舍曲林。在这方面，聚（3,4-亚乙基二氧噻吩）-氧化石墨烯（PEDOT-GO）被电沉积在 SPME 纤维上作为导电涂层，然后纤维在萃取过程中充当接受电极。因此，在电场的影响下，分析物的迁移和它们在纤维涂层上的吸收同时完成。在优化条件下，使用气相色谱-质谱法，目标分析物的检出限在 0.005-0.025μg L 范围内。该方法的线性范围为 0.010-500μg L 用于丙咪嗪和舍曲林，0.025-500μg L 用于阿米替林、去甲替林和去甲丙咪嗪，1.000-250μg L 用于马普替林（R≥0.9984）。此外，该方法提供了合适的精密度和纤维间重现性，RSDs≤8.4%。最终考察了该方法从人骨髓抽吸物、尿液、血浆和井水样品中提取药物的适用性，得到了令人满意的相对回收率，为 93-105%。
