Haseloff Reiner F, Krause Eberhard, Bigl Marina, Mikoteit Kerstin, Stanimirovic Danica, Blasig Ingolf E
Institute of Molecular Pharmacology, Berlin, Germany.
Proteomics. 2006 Mar;6(6):1803-9. doi: 10.1002/pmic.200500182.
Cerebral ischemia causes functional alteration of the blood-brain barrier, formed by brain capillary endothelial cells (BCEC). Changes in protein expression and activity of selected differentially expressed enzymes were investigated in BCEC subjected to hypoxia (24 h) alone or followed by a 24-h reoxygenation. BCEC proteins were isolated, separated by 2-DE, and identified by MALDI-MS. Computer-based 2-D gel analysis identified 21 up-regulated proteins and 4 down-regulated proteins after hypoxia alone and 9 proteins that were further up-regulated after posthypoxic reoxygenation. The expression of the majority of hypoxia-induced proteins was reduced toward control levels during reoxygenation. The most prominent changes were identified for glycolytic enzymes (e.g., phosphoglycerate kinase), proteins of the ER (e.g., calreticulin), and cytoskeletal (e.g., vimentin) proteins. The results indicate that BCEC respond to hypoxia/reoxygenation by adaptive up-regulation of proteins involved in the glycolysis, protein synthesis, and stress response.
脑缺血会导致由脑毛细血管内皮细胞（BCEC）形成的血脑屏障功能改变。研究了单独缺氧（24小时）或随后再进行24小时复氧处理的BCEC中选定的差异表达酶的蛋白质表达和活性变化。分离BCEC蛋白质，通过二维电泳（2-DE）进行分离，并通过基质辅助激光解吸电离质谱（MALDI-MS）进行鉴定。基于计算机的二维凝胶分析确定，单独缺氧后有21种上调蛋白和4种下调蛋白，缺氧后复氧后又有9种蛋白进一步上调。在复氧过程中，大多数缺氧诱导蛋白的表达向对照水平降低。在糖酵解酶（如磷酸甘油酸激酶）、内质网蛋白（如钙网蛋白）和细胞骨架蛋白（如波形蛋白）中发现了最显著的变化。结果表明，BCEC通过对参与糖酵解、蛋白质合成和应激反应的蛋白质进行适应性上调来应对缺氧/复氧。
