Liang Feng, Wang Yun-fang, Nan Xue, Yue Hui-min, Xu Ying-xin, Shi Shuang-shuang, Li Rong, Pei Xue-tao
Department of Stem Cell and Regenerative Medicine, Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005 Dec;27(6):665-9.
To study differentiation of human bone marrow-derived mesenchymal stem cells (BDMSC) into blood vessel endothelial cells for ideal cell origin of complex organ tissue engineering vascularization and injured tissue repairing by cell transplantation.
After different days of induction with vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in 3D fibrin-gels and matrigel, BDMSC and angiogenesis were determined by the utilization of morphological observation, tissue section and CD34, CD31, vascular endothelial growth factor receptor-1 (VEGFR-1, Flt-1), VEGFR-2 (Flk-1), and vWF that were special for blood vessel endothelial cells.
After 3D-cultured and induced with VEGF and bFGF in vitro in fibrin-gels and matrigel for 3-21 days, BDMSC expressed CD34, CD31, Flt-1, Flk-1, and vWF came into vessel-like configuration.
VEGF, bFGF as well as Flt-1 and Flk-1, expressed by BDMSC, may form a feasible microenviroment after induction and play an important role during processes of blood vessel endothelial cell differentiation and vessel-like configuration forming of BDMSC. Mesenchymal stem cells may be applied to tissue engineering vascularization and injured tissue repairing by cell transplantation.
研究人骨髓间充质干细胞（BDMSC）向血管内皮细胞的分化，为复杂器官组织工程血管化及通过细胞移植修复损伤组织寻找理想的细胞来源。
在三维纤维蛋白凝胶和基质胶中用血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）诱导不同天数后，通过形态学观察、组织切片以及利用血管内皮细胞特异性的CD34、CD31、血管内皮生长因子受体-1（VEGFR-1，Flt-1）、VEGFR-2（Flk-1）和vWF来检测BDMSC及血管生成情况。
在纤维蛋白凝胶和基质胶中体外三维培养并用VEGF和bFGF诱导3至21天后，BDMSC表达CD34、CD31、Flt-1、Flk-1，且vWF呈血管样结构。
BDMSC表达的VEGF、bFGF以及Flt-1和Flk-1，诱导后可能形成一个可行的微环境，并在BDMSC向血管内皮细胞分化及形成血管样结构的过程中发挥重要作用。间充质干细胞可应用于组织工程血管化及通过细胞移植修复损伤组织。
