Colazzo Francesca, Alrashed Fahad, Saratchandra Padmini, Carubelli Ivan, Chester Adrian H, Yacoub Magdi H, Taylor Patricia M, Somers Pamela
Heart Science Centre, NHLI, Imperial College London , Harefield, Middlesex , UK and.
Growth Factors. 2014 Oct;32(5):139-49. doi: 10.3109/08977194.2014.945642. Epub 2014 Aug 12.
Herein we combine chemical and mechanical stimulation to investigate the effects of vascular endothelial growth factor (VEGF) and physiological shear stress in promoting the differentiation human adipose derived stem cells (ADSCs) into endothelial cells. ADSCs were isolated and characterized; endothelial differentiation was promoted by culturing confluent cells in 50 ng/ml VEGF under physiological shear stress for up to 14 days. Afterwards, endothelial cells were seeded onto collagen or acellular aortic valve matrices and exposed to four culture conditions: shear stress + VEGF; shear stress - VEGF; static + VEGF and static - VEGF. After 7 days, phenotype was investigated. ADSCs subjected to shear stress and VEGF express a comprehensive range of specific endothelial markers (vWF, eNOS and FLT-1 after 7 days and CD31, FLk-1 and VE-cadherin after 14 days) and maintain the phenotype when seeded onto scaffolds. Our protocol proved to be an efficient source of endothelial-like cells for tissue engineering based on autologous ADSC.
在此，我们结合化学和机械刺激，以研究血管内皮生长因子（VEGF）和生理剪切应力在促进人脂肪来源干细胞（ADSCs）向内皮细胞分化方面的作用。分离并鉴定了ADSCs；通过在生理剪切应力下，将汇合细胞培养在50 ng/ml VEGF中长达14天来促进内皮分化。之后，将内皮细胞接种到胶原蛋白或去细胞主动脉瓣基质上，并暴露于四种培养条件下：剪切应力 + VEGF；剪切应力 - VEGF；静态 + VEGF和静态 - VEGF。7天后，研究细胞表型。经受剪切应力和VEGF的ADSCs表达一系列广泛的特异性内皮标记物（7天后为vWF、eNOS和FLT-1，14天后为CD31、FLk-1和VE-钙黏蛋白），并且接种到支架上时保持该表型。我们的方案被证明是基于自体ADSC的组织工程中内皮样细胞的有效来源。
