大肠杆菌ExbD蛋白的膜拓扑结构。
Membrane topology of the Escherichia coli ExbD protein.
作者信息
Kampfenkel K, Braun V
机构信息
Universität Tübingen, Germany.
出版信息
J Bacteriol. 1992 Aug;174(16):5485-7. doi: 10.1128/jb.174.16.5485-5487.1992.
Abstract
The ExbD protein is involved in the energy-coupled transport of ferric siderophores, vitamin B12, and B-group colicins across the outer membrane of Escherichia coli. In order to study ExbD membrane topology, ExbD-beta-lactamase fusion proteins were constructed. Cells expressing beta-lactamase fusions to residues 53, 57, 70, 76, 78, 80, 92, 121, and 134 of ExbD displayed high levels of ampicillin resistance, whereas fusions to residues 9 and 19 conferred no ampicillin resistance. It is concluded that the only hydrophobic segment of ExbD, encompassing residues 23 to 43, forms a transmembrane domain and that residues 1 to 22 are located in the cytoplasm and residues 44 to 141 are located in the periplasm.
摘要
ExbD蛋白参与铁载体、维生素B12和B族大肠杆菌素跨大肠杆菌外膜的能量偶联转运。为了研究ExbD的膜拓扑结构，构建了ExbD-β-内酰胺酶融合蛋白。表达ExbD第53、57、70、76、78、80、92、121和134位残基与β-内酰胺酶融合蛋白的细胞表现出高水平的氨苄青霉素抗性，而与第9和19位残基的融合蛋白则不具有氨苄青霉素抗性。得出的结论是，ExbD唯一的疏水片段（包含第23至43位残基）形成一个跨膜结构域，第1至22位残基位于细胞质中，第44至141位残基位于周质中。
相似文献
1
Membrane topology of the Escherichia coli ExbD protein.
J Bacteriol. 1992 Aug;174(16):5485-7. doi: 10.1128/jb.174.16.5485-5487.1992.
2
Energy-coupled transport across the outer membrane of Escherichia coli: ExbB binds ExbD and TonB in vitro, and leucine 132 in the periplasmic region and aspartate 25 in the transmembrane region are important for ExbD activity.
J Bacteriol. 1996 May;178(10):2836-45. doi: 10.1128/jb.178.10.2836-2845.1996.
3
Topology of the ExbB protein in the cytoplasmic membrane of Escherichia coli.
J Biol Chem. 1993 Mar 15;268(8):6050-7.
4
Ferric citrate transport of Escherichia coli: functional regions of the FecR transmembrane regulatory protein.
J Bacteriol. 1998 May;180(9):2387-94. doi: 10.1128/JB.180.9.2387-2394.1998.
5
Evolutionary relationship of uptake systems for biopolymers in Escherichia coli: cross-complementation between the TonB-ExbB-ExbD and the TolA-TolQ-TolR proteins.
Mol Microbiol. 1993 Apr;8(2):261-8. doi: 10.1111/j.1365-2958.1993.tb01570.x.
6
Membrane topologies of the TolQ and TolR proteins of Escherichia coli: inactivation of TolQ by a missense mutation in the proposed first transmembrane segment.
J Bacteriol. 1993 Jul;175(14):4485-91. doi: 10.1128/jb.175.14.4485-4491.1993.
7
Transmembrane topology of the two FhuB domains representing the hydrophobic components of bacterial ABC transporters involved in the uptake of siderophores, haem and vitamin B12.
Microbiology (Reading). 1998 Oct;144 ( Pt 10):2759-2769. doi: 10.1099/00221287-144-10-2759.
8
Energy-coupled transport and signal transduction through the gram-negative outer membrane via TonB-ExbB-ExbD-dependent receptor proteins.
FEMS Microbiol Rev. 1995 Jul;16(4):295-307. doi: 10.1111/j.1574-6976.1995.tb00177.x.
9
The solution structure of the periplasmic domain of the TonB system ExbD protein reveals an unexpected structural homology with siderophore-binding proteins.
Mol Microbiol. 2007 Nov;66(4):872-89. doi: 10.1111/j.1365-2958.2007.05957.x. Epub 2007 Oct 10.
10
Colicin M is inactivated during import by its immunity protein.
Mol Gen Genet. 1996 Jun 12;251(3):388-96. doi: 10.1007/BF02172531.
引用本文的文献
1
Substrate Uptake by TonB-Dependent Outer Membrane Transporters.
Mol Microbiol. 2024 Dec;122(6):929-947. doi: 10.1111/mmi.15332. Epub 2024 Dec 3.
2
Radiation-Tolerant spp. from Rhizosphere Soil: Genome Insights and Potential in Agriculture.
Genes (Basel). 2024 Aug 9;15(8):1048. doi: 10.3390/genes15081048.
3
Energization of Outer Membrane Transport by the ExbB ExbD Molecular Motor.
J Bacteriol. 2023 Jun 27;205(6):e0003523. doi: 10.1128/jb.00035-23. Epub 2023 May 23.
4
Bacterial Siderophores: Classification, Biosynthesis, Perspectives of Use in Agriculture.
Plants (Basel). 2022 Nov 12;11(22):3065. doi: 10.3390/plants11223065.
5
Transcription regulation of iron carrier transport genes by ECF sigma factors through signaling from the cell surface into the cytoplasm.
FEMS Microbiol Rev. 2022 Jul 1;46(4). doi: 10.1093/femsre/fuac010.
6
The Intrinsically Disordered Region of ExbD Is Required for Signal Transduction.
J Bacteriol. 2020 Mar 11;202(7). doi: 10.1128/JB.00687-19.
7
Transcriptional responses of Xanthomonas oryzae pv. oryzae to type III secretion system inhibitor ortho-coumaric acid.
BMC Microbiol. 2019 Jul 15;19(1):163. doi: 10.1186/s12866-019-1532-5.
8
Confined Mobility of TonB and FepA in Escherichia coli Membranes.
PLoS One. 2016 Dec 9;11(12):e0160862. doi: 10.1371/journal.pone.0160862. eCollection 2016.
9
Time-resolved pathogenic gene expression analysis of the plant pathogen Xanthomonas oryzae pv. oryzae.
BMC Genomics. 2016 May 10;17:345. doi: 10.1186/s12864-016-2657-7.
10
Membrane Protein Complex ExbB4-ExbD1-TonB1 from Escherichia coli Demonstrates Conformational Plasticity.
J Bacteriol. 2015 Jun;197(11):1873-85. doi: 10.1128/JB.00069-15. Epub 2015 Mar 23.
本文引用的文献
1
Transport of vitamin B12 in Escherichia coli. Some observations on the roles of the gene products of BtuC and TonB.
J Biol Chem. 1980 May 10;255(9):4313-9.
2
Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903.
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7176-80. doi: 10.1073/pnas.77.12.7176.
3
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
4
Complementation analysis of temperature-sensitive host specificity mutations in Escherichia coli.
J Mol Biol. 1970 May 28;50(1):111-27. doi: 10.1016/0022-2836(70)90108-7.
5
A vector for the construction of translational fusions to TEM beta-lactamase and the analysis of protein export signals and membrane protein topology.
Gene. 1986;49(3):341-9. doi: 10.1016/0378-1119(86)90370-7.
6
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
J Mol Biol. 1986 May 5;189(1):113-30. doi: 10.1016/0022-2836(86)90385-2.
7
Suppression of the btuB451 mutation by mutations in the tonB gene suggests a direct interaction between TonB and TonB-dependent receptor proteins in the outer membrane of Escherichia coli.
Gene. 1988 Apr 15;64(1):147-53. doi: 10.1016/0378-1119(88)90488-x.
8
A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8. doi: 10.1073/pnas.82.4.1074.
9
A conformational preference parameter to predict helices in integral membrane proteins.
Biochim Biophys Acta. 1986 Jan 30;869(2):197-214. doi: 10.1016/0167-4838(86)90295-5.
10
Involvement of ExbB and TonB in transport across the outer membrane of Escherichia coli: phenotypic complementation of exb mutants by overexpressed tonB and physical stabilization of TonB by ExbB.
J Bacteriol. 1989 Sep;171(9):5127-34. doi: 10.1128/jb.171.9.5127-5134.1989.
Zotero 插件