Sakae T, Davies J E
Department of Anatomy, Nihon University School of Dentistry, Chiba, Japan.
J Biol Buccale. 1992 Jun;20(2):129-33.
A new quick infrared spectroscopic method was developed and applied to analyze precipitates formed by cultured cells on a Petri dish. This IR method allows a dish to be placed directly on the sample beam window of a normal double beam IR spectrometer. Placing a blank dish on the reference beam window of the spectrometer compensates for the high background absorption due to the dish on the sample beam window and enables an identifiable spectrum to be obtained. Using this technique with a Petri dish, a high correlation factor of 0.998 was found between the PO4 peak absorbance and the weight of synthetic hydroxyapatite in the range of 0.5-2.5 mg/cm2. The IR pattern, however, showed a shift in the positions of the absorption band and the appearance of ghost peaks. Using a thin based Petriperm dish, these difficulties were overcome producing a correlation factor of 0.992 at the lower range of 0.05-1.45 mg/cm2. Application of this technique to the precipitates formed on a Petri dish by rat bone marrow derived cells showed the presence of collagen and apatite-like substance.
开发了一种新的快速红外光谱方法，并将其应用于分析培养细胞在培养皿上形成的沉淀物。这种红外方法允许将培养皿直接放置在普通双光束红外光谱仪的样品光束窗口上。在光谱仪的参比光束窗口放置一个空白培养皿，可以补偿由于样品光束窗口上的培养皿导致的高背景吸收，并能够获得可识别的光谱。使用这种培养皿技术，在0.5 - 2.5 mg/cm²范围内，发现PO4峰吸光度与合成羟基磷灰石重量之间的相关系数高达0.998。然而，红外图谱显示吸收带位置发生了偏移，并出现了鬼峰。使用薄底的Petriperm培养皿，克服了这些困难，在0.05 - 1.45 mg/cm²的较低范围内产生了0.992的相关系数。将该技术应用于大鼠骨髓来源细胞在培养皿上形成的沉淀物，结果表明存在胶原蛋白和类磷灰石物质。
