Johnson-White Brandy, Buquo Lauren, Zeinali Mazyar, Ligler Frances S
Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USA.
Anal Chem. 2006 Feb 1;78(3):853-7. doi: 10.1021/ac051700v.
The ability of Vaccinum macrocarpon, the North American cranberry, to prevent bacterial adhesion has been used to advantage in the prevention of urinary tract infections and has recently been described for the prevention of adhesion of bacteria responsible for oral infections and stomach ulcers. This report documents the ability of cranberry juice to reduce nonspecific adhesion of bacteria to the borosilicate glass microscope slides used in an immunoarray biosensor format. Nonspecific binding of analytes in the array sensor leads to high background signals that cause increased detection limits and false positives. Reduction in background-to-signal ratios can be seen as the juice concentration is increased from 0 to 50% of the sample. This impact cannot be duplicated with grape, orange, apple, or white cranberry juice. Sugar content and pH have been eliminated as the agents in the juice responsible for the anti-adhesive activity.
北美蔓越莓（Vaccinum macrocarpon）预防细菌黏附的能力已被用于预防尿路感染，最近也被描述用于预防引起口腔感染和胃溃疡的细菌黏附。本报告记录了蔓越莓汁降低细菌与免疫阵列生物传感器形式中使用的硼硅酸盐玻璃显微镜载玻片非特异性黏附的能力。阵列传感器中分析物的非特异性结合会导致高背景信号，从而导致检测限增加和假阳性。当果汁浓度从样品的0%增加到50%时，可以看到背景信号与信号的比率降低。葡萄汁、橙汁、苹果汁或白蔓越莓汁无法产生这种效果。已排除果汁中的糖分和pH值是导致抗黏附活性的因素。
