Camps Jordi, Salaverria Itziar, Garcia Maria J, Prat Esther, Beà Sílvia, Pole Jessica C, Hernández Lluis, Del Rey Javier, Cigudosa Juan Cruz, Bernués Marta, Caldas Carlos, Colomer Dolors, Miró Rosa, Campo Elías
Departament de Biologia Cellular, Fisiologia i Immunologia and Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Leuk Res. 2006 Aug;30(8):923-34. doi: 10.1016/j.leukres.2005.11.013. Epub 2006 Jan 31.
Mantle-cell lymphoma (MCL) is genetically characterized by 11q13 chromosomal translocations involving the CCND1 gene. We have characterized five MCL cell lines, JVM-2, GRANTA-519, REC-1, JEKO-1, and NCEB-1, combining metaphase and array comparative genomic hybridization, multicolor-FISH, and molecular analysis. Our results revealed common gained regions at 2p14, 9q31.2-qter, 11q13.1-q21, 13q14-q21.2, 13q34-qter and 18q21.1-q22.1, and losses at 1p21.2-p31.1, 2p11.2, 8p21.2-pter, 9p21.3-pter, 11q23.3-qter, 17p11.2-pter, and 17q21.2-q22.2. All cell lines except JVM-2, displayed moderate or high numerical chromosome instability. In addition, an ongoing level of chromosome rearrangements was observed in REC-1. Surprisingly, NCEB-1 carried several stable mouse chromosomes and showed expression of both human and murine bcl-2 protein. Our findings indicate that these cell lines represent three patterns of chromosome evolution in MCL and may be useful to understand the pathogenesis of this neoplasm.
套细胞淋巴瘤（MCL）的遗传学特征是涉及CCND1基因的11q13染色体易位。我们结合中期和阵列比较基因组杂交、多色荧光原位杂交及分子分析，对5种MCL细胞系JVM-2、GRANTA-519、REC-1、JEKO-1和NCEB-1进行了特征分析。我们的结果揭示了在2p14、9q31.2 - qter、11q13.1 - q21、13q14 - q21.2、13q34 - qter和18q21.1 - q22.1存在常见的获得区域，以及在1p21.2 - p31.1、2p11.2、8p21.2 - pter、9p21.3 - pter、11q23.3 - qter、17p11.2 - pter和17q21.2 - q22.2存在缺失区域。除JVM-2外，所有细胞系均表现出中度或高度的染色体数目不稳定性。此外，在REC-1中观察到持续的染色体重排水平。令人惊讶的是，NCEB-1携带了几条稳定的小鼠染色体，并同时表达人源和鼠源bcl-2蛋白。我们的研究结果表明，这些细胞系代表了MCL中染色体进化的三种模式，可能有助于理解这种肿瘤的发病机制。
