高性能免疫亲和色谱中的多种配体应用

Multiple ligand applications in high-performance immunoaffinity chromatography.

作者信息

Wheatley J B

机构信息

Rainin Instrument Company, Berkeley, CA 94710.

出版信息

J Chromatogr. 1992 Jun 19;603(1-2):273-8. doi: 10.1016/0021-9673(92)85371-y.

DOI:10.1016/0021-9673(92)85371-y

PMID:1644884

Abstract

A mixture of antibodies specific for albumin and transferrin, immobilized onto a single support, was used for the simultaneous extraction of albumin and transferrin from immunoglobulin G by high-performance immunoaffinity chromatography. The affinity column was coupled to a strong cation exchanger in order to monitor the success of the extraction and to demonstrate the compatibility of the two chromatographic modes. Coupling of non-affinity chromatography with multiple ligand affinity chromatography is discussed as an alternative to positive affinity chromatography for protein purification.

摘要

将针对白蛋白和转铁蛋白的特异性抗体混合物固定在单一载体上，通过高效免疫亲和色谱法从免疫球蛋白G中同时提取白蛋白和转铁蛋白。亲和柱与强阳离子交换剂相连，以监测提取的成功与否，并证明两种色谱模式的兼容性。讨论了将非亲和色谱与多配体亲和色谱联用作为蛋白质纯化中正向亲和色谱的替代方法。