Zhang Zheng-Bin, Liu Chun-Ying, Wu Zhen-Zhen, Xing Lei, Li Pei-Feng
Institute of Marine Chemistry, Ocean University of China, Qingdao, China.
Med Sci Monit. 2006 Feb;12(2):BR75-85. Epub 2006 Jan 26.
Multiple functions of nitric oxide (NO) in organisms and its special function and role in the atmosphere were investigated. In this study, marine microalga culture-medium NO concentrations were detected and studied in order to find the laws governing NO release by marine microalgae and relevant material was collected for discussion on the production mechanisms.
MATERIAL/METHODS: NO concentrations in culture media of the marine microalgae Platymonas subcordiformis, Skeletonema costatum, and Gymnodinium sp. were detected and other media parameters, such as nutrients concentrations, Chl-a, alga cell density, and pH, were also measured concurrently.
The NO concentrations in culture media of the marine microalgae Platymonas subcordiformis, Skeletonema costatum, and Gymnodinium sp. were detected and found to be about 10(-8)-10(-9) mol/l. The relationships between NO and nutrients and NO and pH were discussed. It was found that "NO" could serve as the message factor of microalga growth status. Experiments showed that factors affecting alga growth, such as trace elements, light, temperature, and salinity, all affected the culture-medium NO concentration. At the same time, an environmental stimulus could give rise to sudden NO peaks, with NO being a signal molecule of marine microalga stress response.
These results indicated that low concentrations of NO were produced by marine phytoplankton (microalgae) under the condition of normal growth. NO is a message factor of microalga growth and is also a signal molecule of stress response.
研究了一氧化氮（NO）在生物体中的多种功能及其在大气中的特殊功能和作用。本研究检测并研究了海洋微藻培养基中的NO浓度，以找出海洋微藻释放NO的规律，并收集相关材料讨论其产生机制。
材料/方法：检测了亚心形扁藻、中肋骨条藻和裸甲藻等海洋微藻培养基中的NO浓度，并同时测量了其他培养基参数，如营养物质浓度、叶绿素a、藻细胞密度和pH值。
检测了亚心形扁藻、中肋骨条藻和裸甲藻等海洋微藻培养基中的NO浓度，发现其浓度约为10^(-8)-10^(-9)mol/L。讨论了NO与营养物质以及NO与pH值之间的关系。发现“NO”可作为微藻生长状态的信息因子。实验表明，影响藻类生长的因素，如微量元素、光照、温度和盐度，均会影响培养基中的NO浓度。同时，环境刺激可导致NO突然峰值出现，NO是海洋微藻应激反应的信号分子。
这些结果表明，海洋浮游植物（微藻）在正常生长条件下会产生低浓度的NO。NO是微藻生长的信息因子，也是应激反应的信号分子。
