Guo Zhen, Chen Zhaoan, Zhang Wei, Yu Xingju, Jin Meifang
Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Biotechnol Lett. 2008 May;30(5):877-83. doi: 10.1007/s10529-008-9637-1. Epub 2008 Jan 15.
To develop an integrated process of CO(2)-fixation and H(2) photoproduction by marine green microalga Platymonas subcordiformis, the impact of algal cells grown in CO(2)-supplemented air bubble column bioreactor was investigated on H(2) photoproduction regulated by carbonylcyanide m-chlorophenylhrazone. Highest cell growth (3.85 x 10(6) cells ml(-1)), starch content (0.25 +/- 0.08 mg per 10(6)cells) and hydrogen production (50 +/- 3 ml l(-1)) were achieved at 3% CO(2)-supplemented culture, which are respectively 1.4, 2.1, 1.5-fold of the air-supplemented culture. Improved H(2) production correlated well with the increase in starch accumulation. In this process, the algal cells have been recycled for stable H(2) production of 40-50 ml l(-1) over five cycles.
为了开发一种由海洋绿藻亚心形扁藻进行二氧化碳固定和光产氢的整合工艺，研究了在补充二氧化碳的气泡柱生物反应器中培养的藻类细胞对羰基氰化物间氯苯腙调节的光产氢的影响。在补充3%二氧化碳的培养条件下，实现了最高的细胞生长（3.85×10⁶个细胞/毫升）、淀粉含量（每10⁶个细胞0.25±0.08毫克）和产氢量（50±3毫升/升），分别是补充空气培养的1.4倍、2.1倍和1.5倍。产氢量的提高与淀粉积累的增加密切相关。在此过程中，藻类细胞经过五个循环被循环利用，以稳定产生40 - 50毫升/升的氢气。
