Zourob Mohammed, Mohr Stephan, Mayes Andrew G, Macaskill Alexandra, Pérez-Moral Natalia, Fielden Peter R, Goddard Nicholas J
School of Chemical Engineering and Analytical Science, The University of Manchester, PO Box 88, Sackville Street, Manchester M60 1QD, UK.
Lab Chip. 2006 Feb;6(2):296-301. doi: 10.1039/b513195b. Epub 2006 Jan 10.
In this study, uniform spherical molecularly imprinted polymer beads were prepared via controlled suspension polymerization in a spiral-shaped microchannel using mineral oil and perfluorocarbon liquid as continuous phases. Monodisperse droplets containing the monomers, template, initiator, and porogenic solvent were introduced into the microchannel, and particles of uniform size were produced by subsequent UV polymerization, quickly and without wasting polymer materials. The droplet/particle size was varied by changing the flow conditions in the microfluidic device. The diameter of the resulting products typically had a coefficient of variation (CV) below 2%. The specific binding sites that were created during the imprinting process were analysed via radioligand binding analysis. The molecularly imprinted microspheres produced in the liquid perfluorocarbon continuous phase had a higher binding capacity compared with the particles produced in the mineral oil continuous phase, though it should be noted that the aim of this study was not to optimize or maximize imprinting performance, but rather to demonstrate broad applicability and compatibility with known MIP production methods. The successful imprinting against a model compound using two very different continuous phases (one requiring a surfactant to stabilize the droplets the other not) demonstrates the generality of this current simple approach.
在本研究中，通过在螺旋形微通道中以矿物油和全氟化碳液体作为连续相进行可控悬浮聚合，制备了均匀的球形分子印迹聚合物微珠。将含有单体、模板、引发剂和成孔溶剂的单分散液滴引入微通道，随后通过紫外光聚合快速生成尺寸均匀的颗粒，且不浪费聚合物材料。通过改变微流控装置中的流动条件来改变液滴/颗粒的大小。所得产物的直径通常变异系数（CV）低于2%。通过放射性配体结合分析对印迹过程中产生的特异性结合位点进行了分析。与在矿物油连续相中制备的颗粒相比，在全氟化碳液体连续相中制备的分子印迹微球具有更高的结合能力，不过需要注意的是，本研究的目的不是优化或最大化印迹性能，而是展示其广泛的适用性以及与已知分子印迹聚合物生产方法的兼容性。使用两种截然不同的连续相（一种需要表面活性剂来稳定液滴，另一种则不需要）成功地对模型化合物进行印迹，证明了这种当前简单方法的通用性。
