Kempe Henrik, Kempe Maria
Biomedical Polymer Technology, Department of Experimental Medical Science, Biomedical Center, Lund University, SE-221 84 Lund, Sweden.
Anal Chem. 2006 Jun 1;78(11):3659-66. doi: 10.1021/ac060068i.
The majority of studies on molecularly imprinted polymers has until now been carried out on irregularly shaped particles prepared by grinding of polymer monoliths. The preparation procedures are time- and labor-consuming and produce particles of wide size distributions. To answer the need for fast and straightforward routes to spherical molecularly imprinted polymer beads, we have developed a method comprising the formation of droplets of pre-polymerization solution directly in mineral oil by vigorous mixing followed by transformation of the droplets into solid spherical beads by photoinduced free-radical polymerization. No detergents or stabilizers were required for the droplet formation. Factors influencing the bead synthesis have been investigated and are detailed here. The beads were evaluated in parallel with corresponding irregularly shaped particles prepared from polymer monoliths. Conditions for the synthesis of propranolol-imprinted poly(methacrylic acid-co-trimethylolpropane trimethacrylate) beads in the size range of 1-100 microm in almost quantitative yield are described. The beads were applied as the recognition element in a 96-well plate format radioligand assay of propranolol in human serum.
到目前为止，大多数关于分子印迹聚合物的研究都是在通过研磨聚合物整体制备的不规则形状颗粒上进行的。制备过程既耗时又费力，并且会产生尺寸分布很宽的颗粒。为了满足对快速、直接制备球形分子印迹聚合物珠的需求，我们开发了一种方法，该方法包括通过剧烈混合在矿物油中直接形成预聚合溶液的液滴，然后通过光引发自由基聚合将液滴转化为固体球形珠。液滴形成过程无需洗涤剂或稳定剂。已对影响珠合成的因素进行了研究，并在此详细说明。将这些珠与由聚合物整体制备的相应不规则形状颗粒进行了平行评估。描述了以几乎定量的产率合成尺寸范围为1 - 100微米的普萘洛尔印迹聚（甲基丙烯酸 - 共 - 三羟甲基丙烷三甲基丙烯酸酯）珠的条件。这些珠被用作96孔板形式的人血清中普萘洛尔放射性配体测定的识别元件。
