Mushipe Moses T, Chen Xi, Jennings David, Li Gang
Musculoskeletal Education and Research Unit, School of Medicine and Dentistry, Queen's University Belfast, Musgrave Park Hospital, Belfast, BT9 7JB, United Kingdom.
J Orthop Res. 2006 Mar;24(3):501-7. doi: 10.1002/jor.20044.
The objective of this study was to investigate the potential application of cell-seeded biomaterials for revision arthroplasty and the reconstruction of major joints using the impaction grafting technique. Using morselized cancellous bone graft as a porous scaffold, MG63 cells were seeded on the scaffold and impacted into an acetabulum cup model using a mechanical device constructed from data obtained during impaction grafting by an orthopedic surgeon. Immediately after impaction, cells were trypsinized from the scaffold and processed for cell survival rates using the double-stranded DNA PicoGreen assay. Significant reductions in viable cells were observed between the fifth impact and both the first and second impacts (p < 0.01 and p < 0.05, respectively). Cell survival rate was 21.5% after five impacts. The biological performance of cell-seeded biomaterials may be enhanced by these surviving cells. Compared to allograft bone that is not osteogenic, a cell-seeded biomaterial might also be a suitable substitute for allograft bone for major joint reconstruction at revision arthroplasty.
本研究的目的是探讨接种细胞的生物材料在翻修关节成形术及使用打压植骨技术重建大关节方面的潜在应用。以松质骨碎骨移植作为多孔支架，将MG63细胞接种于支架上，并使用由骨科医生在打压植骨过程中获取的数据构建的机械设备将其打入髋臼杯模型。打压后立即将细胞从支架上用胰蛋白酶消化，并使用双链DNA PicoGreen检测法检测细胞存活率。在第五次打压与第一次和第二次打压之间观察到活细胞显著减少（分别为p < 0.01和p < 0.05）。五次打压后细胞存活率为21.5%。这些存活的细胞可能会增强接种细胞的生物材料的生物学性能。与无成骨能力的同种异体骨相比，接种细胞的生物材料也可能是翻修关节成形术时用于大关节重建的同种异体骨的合适替代品。
