Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
EMBO J. 1983;2(5):691-6. doi: 10.1002/j.1460-2075.1983.tb01486.x.
The essential sequences needed for Alu repeat transcription by RNA polymerase III were mapped. Experimental evidence is presented showing that the Alu repeat promoters are organised in a bipartite structure similar to the split tRNA promoters as suggested by DNA sequence homology. Furthermore, by combining fragments from efficiently and inefficiently transcribed natural Alu repeats in several recombinant clones, it was possible to investigate the regions responsible for their differences. It is clear that, apart from the short stretches of homology with the tRNA consensus sequence, there is very little constraint in the promoter sequences. However, our studies indicate that some influence on the efficiency of transcription may be exerted by regions outside the accepted promoter components.
已经对 RNA 聚合酶 III 转录 Alu 重复序列所需的基本序列进行了定位。实验证据表明，Alu 重复序列启动子组织成双份结构，类似于 DNA 序列同源性所暗示的分裂 tRNA 启动子。此外，通过在几个重组克隆中组合来自有效和低效转录的天然 Alu 重复序列的片段，有可能研究负责它们差异的区域。很明显，除了与 tRNA 共有序列的短同源序列外，启动子序列几乎没有限制。然而，我们的研究表明，某些区域可能会对转录效率产生影响，这些区域不在公认的启动子成分内。
