Galli G, Hofstetter H, Birnstiel M L
Nature. 1981 Dec 17;294(5842):626-31. doi: 10.1038/294626a0.
The split promoter sequences of a tRNALeuCUG gene of Xenopus laevis have been mapped to nucleotides 13-20 and 51-64 of the tRNALeu coding sequences. The sequences closely coincide with two conserved sequence blocks present in all eukaryotic tRNA genes. The two conserved sequence blocks were found to be exchangeable between tRNA genes as chimaeric tRNAMet--tRNALeu genes proved transcriptionally active. Furthermore, two prokaryotic tRNA genes exhibiting strong homologies with the two blocks yielded specific transcripts when tested in an eukaryotic transcriptional system.
非洲爪蟾tRNALeuCUG基因的分裂启动子序列已被定位到tRNALeu编码序列的第13 - 20位核苷酸和第51 - 64位核苷酸。这些序列与所有真核tRNA基因中存在的两个保守序列块紧密重合。由于嵌合的tRNAMet - tRNALeu基因被证明具有转录活性，所以发现这两个保守序列块在tRNA基因之间是可交换的。此外，与这两个序列块表现出强烈同源性的两个原核tRNA基因，在真核转录系统中进行测试时产生了特异性转录本。
