Depicker A, Stachel S, Dhaese P, Zambryski P, Goodman H M
J Mol Appl Genet. 1982;1(6):561-73.
The DNA sequence of the nopaline synthase gene (nos) from Agrobacterium tumefaciens Ti plasmid pTiT37 and adjacent regions up to the right border of the T-DNA was determined. The 5' and 3' termini of the polyadenylated nos mRNA, isolated from a T37 tobacco teratoma tumor line, were localized by S1 mapping. The final mRNA is unspliced, encoded by a region of about 1450 bp, and specifies an open reading frame of 413 amino acids. Potential transcriptional signals in the 5' flanking DNA, such as CATAAA ("TATA box") and GGTCACTAT ("CAT box"), bear close resemblance to other eukaryotic promoters. Two putative polyadenylation signals, AATAAA and AATAAT, are found about 135 and 50 bp from the 3' end, respectively. This study may provide information for the development of expression vectors for genes in plant cells; moreover, the structural gene can be used as an easy screenable marker.
测定了根癌农杆菌Ti质粒pTiT37中胭脂碱合成酶基因（nos）的DNA序列以及直至T-DNA右边界的相邻区域。通过S1作图法确定了从T37烟草畸胎瘤肿瘤系中分离出的多聚腺苷酸化nos mRNA的5'和3'末端。最终的mRNA未经过剪接，由一个约1450 bp的区域编码，并指定了一个413个氨基酸的开放阅读框。5'侧翼DNA中的潜在转录信号，如CATAAA（“TATA框”）和GGTCACTAT（“CAT框”），与其他真核启动子极为相似。分别在距3'末端约135和50 bp处发现了两个假定的多聚腺苷酸化信号AATAAA和AATAAT。该研究可为植物细胞中基因表达载体的开发提供信息；此外，该结构基因可用作易于筛选的标记。
