Botanisches Institut der Universität, Universitätsstr. 1, 4 Düsseldorf 1, FRG.
EMBO J. 1983;2(12):2229-37. doi: 10.1002/j.1460-2075.1983.tb01728.x.
A core particle of the water-oxidizing photosystem II reaction center has been prepared from stacked spinach thylakoid membranes by a procedure involving extraction with the non-ionic detergent dodecyl-beta-D-maltoside and centrifugation in sucrose gradients. The protein-pigment complex consists of at least four polypeptide species: two components with mol. wts. of 51 and 44 kd which are conjugated with chlorophyll a and beta-carotene, the herbicide-binding protein of mol. wt. 32 kd and cytochrome b 559 (11 kd). The genes for the 51-and 44-kd polypeptides have been located on the circular 150-kbp spinach plastid chromosome. They were identified by hybrid-selection mapping, in vitro transcription-translation of recombinant DNAs and specific antisera which were used to characterize the translation products. The plastid chromosome carries one uninterrupted copy for each of these genes in its large single-copy region. The gene for the 51-kd protein (which probably bears the P(680) reaction center chlorophyll a) is located in close proximity to the gene for cytochrome b6, and some 70 kbp away from the gene for the ;32-kd' herbicide-binding protein of the reducing side of photosystem II. The gene for the 44-kd protein is situated halfway between these two genes adjacent to the gene for the P(700) chlorophyll a apoprotein of the photosystem I reaction center. Both photosystem II genes are transcribed into discrete RNA species in the same direction but from the opposite strand as the gene for the ;32-kd' protein.
水氧化光合系统 II 反应中心的核心颗粒已由堆叠菠菜类囊体膜通过用非离子去污剂十二烷基-β-D-麦芽糖苷提取和蔗糖梯度离心的程序制备。蛋白质-色素复合物至少由四种多肽组成:两种分子量为 51 和 44 kDa 的组分与叶绿素 a 和β-胡萝卜素结合,分子量为 32 kDa 的除草剂结合蛋白和细胞色素 b 559(11 kDa)。51 和 44 kDa 多肽的基因已定位在圆形 150-kbp 菠菜质体染色体上。通过杂交选择作图、体外转录翻译重组 DNA 和用于表征翻译产物的特异性抗血清鉴定了这些基因。质体染色体在其大片段单拷贝区中携带这些基因的一个不间断拷贝。51 kDa 蛋白(可能带有 P(680)反应中心叶绿素 a)的基因与细胞色素 b6 基因紧密相邻,距离 photosystem II 还原侧的;32-kd' 除草剂结合蛋白基因约 70 kbp。44 kDa 蛋白的基因位于这两个基因之间,紧邻 photosystem I 反应中心 P(700)叶绿素 a 脱辅基蛋白的基因。两个 photosystem II 基因均转录成离散的 RNA 物种,在相同方向但与;32-kd' 蛋白基因的相反链上。