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菠菜叶绿体 ATP 酶β和ε亚基基因的结构表明，该基因是一个双顺反子 mRNA，且存在一个翻译终止/起始信号重叠。

Structures of the genes for the beta and epsilon subunits of spinach chloroplast ATPase indicate a dicistronic mRNA and an overlapping translation stop/start signal.

机构信息

Division of Plant Industry, Commonwealth Scientific and Industrial Research Organisation, Post Office Box 1600, Canberra City, A.C.T. 2601, Australia.

出版信息

Proc Natl Acad Sci U S A. 1982 Oct;79(20):6260-4. doi: 10.1073/pnas.79.20.6260.

DOI:10.1073/pnas.79.20.6260
PMID:16593238
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC347100/
Abstract

A 2,4-kilobase-pair region of spinach chloroplast DNA adjacent to the gene for the large subunit of ribulosebisphosphate carboxylase has been analyzed by RNA hybridization, in vitro transcription/translation, and DNA sequence determination. The analysis indicates that this region carries the genes for the beta and epsilon subunits of chloroplast ATPase and that the two genes are cotranscribed into a dicistronic mRNA with 4-base-pair overlap between the stop codon of the beta-subunit gene and the start codon of the epsilon-subunit gene. The ATPase and carboxylase genes are transcribed divergently with respect to each other. The deduced amino acid sequences of the beta and epsilon subunits from spinach show 67% and 26% homology, respectively, with the published sequences of the beta and epsilon subunits of Escherichia coli ATPase.

摘要

菠菜叶绿体 DNA 紧邻核酮糖二磷酸羧化酶大亚基基因的 2.4 千碱基对区域已通过 RNA 杂交、体外转录/翻译和 DNA 序列测定进行了分析。分析表明，该区域携带叶绿体 ATP 酶的β和ε亚基基因，并且这两个基因被转录成具有 4 个碱基对重叠的二顺反子 mRNA，β-亚基基因的终止密码子和 ε-亚基基因的起始密码子。ATP 酶和羧化酶基因相对于彼此进行差异转录。菠菜的β和ε亚基的推导氨基酸序列分别与大肠杆菌 ATP 酶的β和ε亚基的已公布序列具有 67%和 26%的同源性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b93c/347100/b17cc17ca8e4/pnas00459-0155-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b93c/347100/12ec5afc6e17/pnas00459-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b93c/347100/b17cc17ca8e4/pnas00459-0155-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b93c/347100/12ec5afc6e17/pnas00459-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b93c/347100/b17cc17ca8e4/pnas00459-0155-b.jpg

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Structures of the genes for the beta and epsilon subunits of spinach chloroplast ATPase indicate a dicistronic mRNA and an overlapping translation stop/start signal.菠菜叶绿体 ATP 酶β和ε亚基基因的结构表明，该基因是一个双顺反子 mRNA，且存在一个翻译终止/起始信号重叠。
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本文引用的文献

1
Sizes, locations, and directions of transcription of two genes on a cloned maize chloroplast DNA sequence.两个基因在克隆的玉米叶绿体 DNA 序列上的转录大小、位置和方向。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1832-6. doi: 10.1073/pnas.77.4.1832.
2
Identification of an essential glutamic acid residue in the beta subunit of the adenosine triphosphatase from the thermophilic bacterium PS3.嗜热细菌PS3三磷酸腺苷酶β亚基中一个必需谷氨酸残基的鉴定。
J Biol Chem. 1981 Jan 10;256(1):148-53.
3
Subunits of the adenosine triphosphatase complex translated in vitro from the Escherichia coli unc operon.
Photosynth Res. 1985 Mar;6(1):3-31. doi: 10.1007/BF00029044.
4
Structure, organization and expression of cyanobacterial ATP synthase genes.蓝藻 ATP 合酶基因的结构、组织和表达。
Photosynth Res. 1988 Oct;18(1-2):223-44. doi: 10.1007/BF00042986.
5
The chloroplast genes encoding subunits of the H(+)-ATP synthase.编码 H(+)-ATP 合酶亚基的叶绿体基因。
Photosynth Res. 1988 Oct;18(1-2):205-22. doi: 10.1007/BF00042985.
6
Nicotiana chloroplast genes for components of the photosynthetic apparatus.烟草叶绿体光合作用器官的组成基因。
Photosynth Res. 1988 Oct;18(1-2):7-31. doi: 10.1007/BF00042978.
7
Synthesis of the large subunit of ribulose-1,5-bisphosphate carboxylase in anin vitro partially definedE. coli system.在体外部分定义的大肠杆菌系统中合成核酮糖-1,5-二磷酸羧化酶大亚基。
Plant Mol Biol. 1983 Sep;2(5):279-90. doi: 10.1007/BF01578646.
8
Genes and transcripts for the P700 chlorophylla apoprotein and subunit 2 of the photosystem I reaction center complex from spinach thylakoid membranes.菠菜类囊体膜中 P700 叶绿素 a 脱辅基蛋白和光系统 I 反应中心复合物亚基 2 的基因和转录物。
Plant Mol Biol. 1983 Mar;2(2):95-107. doi: 10.1007/BF01595171.
9
Nucleotide sequences of the genes for the alpha, beta and epsilon subunits of wheat chloroplast ATP synthase.小麦叶绿体 ATP 合酶的α、β和ε亚基基因的核苷酸序列。
Plant Mol Biol. 1985 Nov;4(6):333-45. doi: 10.1007/BF02418255.
10
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Plant Mol Biol. 1985 Mar;4(2-3):169-79. doi: 10.1007/BF02418764.
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Nucleic Acids Res. 1981 Oct 24;9(20):5287-96. doi: 10.1093/nar/9.20.5287.
5
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Nucleic Acids Res. 1981 Jul 24;9(14):3251-70. doi: 10.1093/nar/9.14.3251.
6
A system for shotgun DNA sequencing.一种用于鸟枪法DNA测序的系统。
Nucleic Acids Res. 1981 Jan 24;9(2):309-21. doi: 10.1093/nar/9.2.309.
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Nucleic Acids Res. 1980 Apr 25;8(8):1731-43. doi: 10.1093/nar/8.8.1731.
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J Biol Chem. 1981 Sep 10;256(17):9084-9.