Division of Plant Industry, Commonwealth Scientific and Industrial Research Organisation, Post Office Box 1600, Canberra City, A.C.T. 2601, Australia.
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6260-4. doi: 10.1073/pnas.79.20.6260.
A 2,4-kilobase-pair region of spinach chloroplast DNA adjacent to the gene for the large subunit of ribulosebisphosphate carboxylase has been analyzed by RNA hybridization, in vitro transcription/translation, and DNA sequence determination. The analysis indicates that this region carries the genes for the beta and epsilon subunits of chloroplast ATPase and that the two genes are cotranscribed into a dicistronic mRNA with 4-base-pair overlap between the stop codon of the beta-subunit gene and the start codon of the epsilon-subunit gene. The ATPase and carboxylase genes are transcribed divergently with respect to each other. The deduced amino acid sequences of the beta and epsilon subunits from spinach show 67% and 26% homology, respectively, with the published sequences of the beta and epsilon subunits of Escherichia coli ATPase.
菠菜叶绿体 DNA 紧邻核酮糖二磷酸羧化酶大亚基基因的 2.4 千碱基对区域已通过 RNA 杂交、体外转录/翻译和 DNA 序列测定进行了分析。分析表明，该区域携带叶绿体 ATP 酶的β和ε亚基基因，并且这两个基因被转录成具有 4 个碱基对重叠的二顺反子 mRNA，β-亚基基因的终止密码子和 ε-亚基基因的起始密码子。ATP 酶和羧化酶基因相对于彼此进行差异转录。菠菜的β和ε亚基的推导氨基酸序列分别与大肠杆菌 ATP 酶的β和ε亚基的已公布序列具有 67%和 26%的同源性。
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