Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138, USA.
EMBO J. 1984 Jan;3(1):235-43. doi: 10.1002/j.1460-2075.1984.tb01790.x.
The ecdysone-inducible polypeptides (EIPs) 28, 29 and 40 were identified previously as polypeptides whose synthesis is stimulated early in the ecdysone response of Drosophila Kc cells. We have now shown, using two-dimensional gels, that each of these EIPs consists of three species differing in pI, and all stimulated by ecdysone. Translations and hybrid-arrested translations indicated that the poly(A) EIP mRNAs increase 10-fold in abundance during the first 4 h of ecdysone treatment. By a differential screen of a cDNA library we have identified cDNA clones corresponding to all three EIPs. Two kinds of clones were isolated: one hybridizes to the EIP 40 mRNA(s); the second hybridizes to the mRNA(s) encoding all the EIPs 28 and 29. The EIP 28/29 and EIP 40 loci detected by these clones are each present at single sites on the polytene chromosomes and each is at or in the vicinity of an ecdysone-regulated puff.
蜕皮激素诱导多肽（EIPs）28、29 和 40 先前被鉴定为在果蝇 Kc 细胞的蜕皮激素反应早期合成受到刺激的多肽。我们现在使用二维凝胶表明，这些 EIP 中的每一种都由三种在等电点（pI）上有所不同的物种组成，并且都受到蜕皮激素的刺激。翻译和杂交抑制翻译表明，多聚 A 端的 EIP mRNAs 在蜕皮激素处理的前 4 小时内丰度增加了 10 倍。通过对 cDNA 文库的差异筛选，我们已经鉴定出与所有三种 EIP 相对应的 cDNA 克隆。分离出两种类型的克隆：一种与 EIP 40 mRNA 杂交；第二种与编码所有 EIP 28 和 29 的 mRNA 杂交。这些克隆检测到的 EIP 28/29 和 EIP 40 基因座分别位于多线染色体的单个位点上，并且每个基因座都位于或靠近蜕皮激素调节的膨突附近。
