Buetti E, Diggelmann H
Swiss Institute for Experimental Cancer Research, Epalinges, Lausanne, Switzerland.
EMBO J. 1983;2(8):1423-9. doi: 10.1002/j.1460-2075.1983.tb01601.x.
Transcription of mouse mammary tumor virus (MMTV) DNA is stimulated by steroid hormones. To determine the DNA sequences involved in this regulation, we constructed a plasmid containing the MMTV long terminal repeat (LTR) in front of the coding region of the herpes simplex thymidine kinase gene, from which the promoter had been removed. Portions of the LTR were removed by the nuclease Ba/31, and the deleted molecules were recloned and tested for transcriptional activity in transfections of Ltk-aprt- cells. Stably transfected cell clones were selected and hormone-dependent transcription from the MMTV promoter was studied by the S1 nuclease mapping method. The results show that DNA sequences between -105 and -204 base pairs upstream from the initiation site of viral transcription are required for glucocorticoid stimulation.
小鼠乳腺肿瘤病毒（MMTV）DNA的转录受类固醇激素刺激。为了确定参与这种调控的DNA序列，我们构建了一个质粒，该质粒在单纯疱疹胸苷激酶基因的编码区前含有MMTV长末端重复序列（LTR），其启动子已被去除。用核酸酶Ba/31去除LTR的部分片段，将缺失的分子重新克隆，并在Ltk-aprt-细胞转染中测试其转录活性。选择稳定转染的细胞克隆，并用S1核酸酶作图法研究来自MMTV启动子的激素依赖性转录。结果表明，病毒转录起始位点上游-105至-204碱基对之间的DNA序列是糖皮质激素刺激所必需的。
