Cherbas L, Cherbas P
Department of Biology, Indiana University, Bloomington 47405, USA.
Genetics. 1997 Feb;145(2):349-58. doi: 10.1093/genetics/145.2.349.
Drosophila cells in culture can be transformed by introducing exogenous DNA carrying a selectable marker. Here we report on the fate of plasmids that contain an extended fragment of Drosophila DNA in addition to the selectable marker. A small minority of the resulting transformants appear to arise from homologous recombination at the chromosomal target. However, the majority of the insertions are the products of illegitimate events in the vicinity of the target DNA, and they often cause mutations in the targeted region. The efficiency of this process, its homology dependence, and the clustering of the products define a novel transformation pathway that we call "parahomologous targeting."
培养中的果蝇细胞可通过导入携带选择标记的外源DNA进行转化。在此，我们报告了除选择标记外还包含果蝇DNA延伸片段的质粒的命运。少数所得转化体似乎源自染色体靶位点的同源重组。然而，大多数插入是靶DNA附近非正当事件的产物，并且它们经常在靶向区域引起突变。这一过程的效率、其对同源性的依赖性以及产物的聚集定义了一种我们称为“旁同源靶向”的新型转化途径。
