电鳐乙酰胆碱受体乙酰胆碱结合α亚基的完整mRNA编码序列：多肽链跨膜组织的模型
Complete mRNA coding sequence of the acetylcholine binding alpha-subunit of Torpedo marmorata acetylcholine receptor: a model for the transmembrane organization of the polypeptide chain.
作者信息
Devillers-Thiery A, Giraudat J, Bentaboulet M, Changeux J P
出版信息
Proc Natl Acad Sci U S A. 1983 Apr;80(7):2067-71. doi: 10.1073/pnas.80.7.2067.
Abstract
A 1,350-base-pair-long cDNA clone, named p alpha-2, was isolated by hybridization to the previously characterized clone p alpha-1 and found to be specific for the alpha-subunit of the Torpedo marmorata acetylcholine receptor. The nucleotide sequences of both cDNA inserts were analyzed and the sequence of the complete coding region and part of the 5' and 3' untranslated regions of the alpha-chain mRNA was determined. The complete amino acid sequence of the alpha-chain precursor is presented and used to develop a model for the transmembrane organization of the polypeptide.
摘要
通过与先前鉴定的克隆pα-1杂交，分离出一个长度为1350个碱基对的cDNA克隆，命名为pα-2，发现它对电鳐乙酰胆碱受体的α亚基具有特异性。分析了两个cDNA插入片段的核苷酸序列，并确定了α链mRNA完整编码区以及部分5'和3'非翻译区的序列。给出了α链前体的完整氨基酸序列，并用于构建该多肽跨膜结构的模型。
相似文献
1
Complete mRNA coding sequence of the acetylcholine binding alpha-subunit of Torpedo marmorata acetylcholine receptor: a model for the transmembrane organization of the polypeptide chain.电鳐乙酰胆碱受体乙酰胆碱结合α亚基的完整mRNA编码序列：多肽链跨膜组织的模型
Proc Natl Acad Sci U S A. 1983 Apr;80(7):2067-71. doi: 10.1073/pnas.80.7.2067.
2
Identification of a cDNA clone coding for the acetylcholine binding subunit of Torpedo marmorata acetylcholine receptor.编码斑纹电鳐乙酰胆碱受体乙酰胆碱结合亚基的cDNA克隆的鉴定。
EMBO J. 1982;1(6):713-7. doi: 10.1002/j.1460-2075.1982.tb01235.x.
3
Isolation and characterization of a cDNA clone for the complete protein coding region of the delta subunit of the mouse acetylcholine receptor.小鼠乙酰胆碱受体δ亚基完整蛋白质编码区cDNA克隆的分离与鉴定。
Proc Natl Acad Sci U S A. 1984 Dec;81(24):7970-4. doi: 10.1073/pnas.81.24.7970.
4
Molecular genetics of Torpedo marmorata acetylcholine receptor.
Adv Exp Med Biol. 1984;181:17-29. doi: 10.1007/978-1-4684-4868-9_3.
5
Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor gamma subunit.加州电鳐乙酰胆碱受体γ亚基的核苷酸及推导的氨基酸序列
Proc Natl Acad Sci U S A. 1983 Feb;80(4):1111-5. doi: 10.1073/pnas.80.4.1111.
6
Primary structure of alpha-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequence.从cDNA序列推导的加州电鳐乙酰胆碱受体α亚基前体的一级结构
Nature. 1982 Oct 28;299(5886):793-7. doi: 10.1038/299793a0.
7
Molecular cloning of cDNA coding for the gamma subunit of Torpedo acetylcholine receptor.电鳐乙酰胆碱受体γ亚基编码cDNA的分子克隆
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4466-70. doi: 10.1073/pnas.79.14.4466.
8
Cloning and sequence analysis of calf cDNA and human genomic DNA encoding alpha-subunit precursor of muscle acetylcholine receptor.编码肌肉乙酰胆碱受体α亚基前体的小牛cDNA和人类基因组DNA的克隆与序列分析
Nature. 1983;305(5937):818-23. doi: 10.1038/305818a0.
9
10
The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor.编码乙酰胆碱受体α亚基的cDNA的分子克隆与特性分析。
Nucleic Acids Res. 1982 Oct 11;10(19):5809-22. doi: 10.1093/nar/10.19.5809.
引用本文的文献
1
Wandering about allostery.变构漫游
Biol Direct. 2024 Aug 8;19(1):64. doi: 10.1186/s13062-024-00502-0.
2
Highlighting membrane protein structure and function: A celebration of the Protein Data Bank.突出膜蛋白结构和功能：纪念蛋白质数据库。
J Biol Chem. 2021 Jan-Jun;296:100557. doi: 10.1016/j.jbc.2021.100557. Epub 2021 Mar 18.
3
Interplay between Gating and Block of Ligand-Gated Ion Channels.配体门控离子通道的门控与阻断之间的相互作用
Brain Sci. 2020 Dec 1;10(12):928. doi: 10.3390/brainsci10120928.
4
Structure of the Native Muscle-type Nicotinic Receptor and Inhibition by Snake Venom Toxins.天然肌肉型烟碱型乙酰胆碱受体的结构和蛇毒毒素的抑制作用。
Neuron. 2020 Jun 17;106(6):952-962.e5. doi: 10.1016/j.neuron.2020.03.012. Epub 2020 Apr 9.
5
Progress in nicotinic receptor structural biology.烟碱型乙酰胆碱受体结构生物学研究进展。
Neuropharmacology. 2020 Jul;171:108086. doi: 10.1016/j.neuropharm.2020.108086. Epub 2020 Apr 7.
6
Discovery of the First Neurotransmitter Receptor: The Acetylcholine Nicotinic Receptor.发现第一种神经递质受体：乙酰胆碱烟碱受体。
Biomolecules. 2020 Apr 3;10(4):547. doi: 10.3390/biom10040547.
7
The nicotinic acetylcholine receptor: a typical 'allosteric machine'.烟碱型乙酰胆碱受体：一种典型的“变构机器”。
Philos Trans R Soc Lond B Biol Sci. 2018 Jun 19;373(1749). doi: 10.1098/rstb.2017.0174.
8
The Ascaris suum nicotinic receptor, ACR-16, as a drug target: Four novel negative allosteric modulators from virtual screening.猪蛔虫烟碱受体ACR-16作为药物靶点：虚拟筛选得到的四种新型负变构调节剂
Int J Parasitol Drugs Drug Resist. 2016 Feb 10;6(1):60-73. doi: 10.1016/j.ijpddr.2016.02.001. eCollection 2016 Apr.
9
The concept of allosteric interaction and its consequences for the chemistry of the brain.变构相互作用的概念及其对大脑化学的影响。
J Biol Chem. 2013 Sep 20;288(38):26969-26986. doi: 10.1074/jbc.X113.503375. Epub 2013 Jul 22.
10
The nicotinic acetylcholine receptor: the founding father of the pentameric ligand-gated ion channel superfamily.烟碱型乙酰胆碱受体：五聚体配体门控离子通道超家族的奠基人。
J Biol Chem. 2012 Nov 23;287(48):40207-15. doi: 10.1074/jbc.R112.407668. Epub 2012 Oct 4.
本文引用的文献
1
ON THE NATURE OF ALLOSTERIC TRANSITIONS: A PLAUSIBLE MODEL.关于别构转变的本质：一个合理的模型。
J Mol Biol. 1965 May;12:88-118. doi: 10.1016/s0022-2836(65)80285-6.
2
Reconstitution of a functional acetylcholine receptor. Conservation of the conformational and allosteric transitions and recovery of the permeability response; role of lipids.功能性乙酰胆碱受体的重组。构象和变构转变的保守性以及通透性反应的恢复；脂质的作用。
Eur J Biochem. 1980 Sep;110(1):35-55. doi: 10.1111/j.1432-1033.1980.tb04839.x.
3
Acetylcholine receptor: complex of homologous subunits.乙酰胆碱受体：同源亚基复合体。
Science. 1980 Jun 27;208(4451):1454-6. doi: 10.1126/science.7384786.
4
Membrane proteins: the amino acid composition of membrane-penetrating segments.膜蛋白：膜穿透片段的氨基酸组成。
Eur J Biochem. 1981 Nov;120(2):275-8. doi: 10.1111/j.1432-1033.1981.tb05700.x.
5
6
On the hydrophobic nature of signal sequences.关于信号序列的疏水性本质。
Eur J Biochem. 1981 May 15;116(2):419-22. doi: 10.1111/j.1432-1033.1981.tb05351.x.
7
Reconstitution of acetylcholine receptor function using purified receptor protein.利用纯化的受体蛋白重建乙酰胆碱受体功能。
Biochemistry. 1981 Feb 17;20(4):694-701. doi: 10.1021/bi00507a004.
8
Structure and function of an acetylcholine receptor.乙酰胆碱受体的结构与功能。
Biophys J. 1982 Jan;37(1):371-83. doi: 10.1016/S0006-3495(82)84685-7.
9
Specific labeling of 3' termini with T4 DNA polymerase.使用T4 DNA聚合酶对3'末端进行特异性标记。
Methods Enzymol. 1980;65(1):39-43. doi: 10.1016/s0076-6879(80)65008-3.
10
Molecular cloning of cDNA coding for the gamma subunit of Torpedo acetylcholine receptor.电鳐乙酰胆碱受体γ亚基编码cDNA的分子克隆
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4466-70. doi: 10.1073/pnas.79.14.4466.
Zotero 插件