电鳐乙酰胆碱受体乙酰胆碱结合α亚基的完整mRNA编码序列：多肽链跨膜组织的模型
Complete mRNA coding sequence of the acetylcholine binding alpha-subunit of Torpedo marmorata acetylcholine receptor: a model for the transmembrane organization of the polypeptide chain.
作者信息
Devillers-Thiery A, Giraudat J, Bentaboulet M, Changeux J P
出版信息
Proc Natl Acad Sci U S A. 1983 Apr;80(7):2067-71. doi: 10.1073/pnas.80.7.2067.
Abstract
A 1,350-base-pair-long cDNA clone, named p alpha-2, was isolated by hybridization to the previously characterized clone p alpha-1 and found to be specific for the alpha-subunit of the Torpedo marmorata acetylcholine receptor. The nucleotide sequences of both cDNA inserts were analyzed and the sequence of the complete coding region and part of the 5' and 3' untranslated regions of the alpha-chain mRNA was determined. The complete amino acid sequence of the alpha-chain precursor is presented and used to develop a model for the transmembrane organization of the polypeptide.
摘要
通过与先前鉴定的克隆pα-1杂交，分离出一个长度为1350个碱基对的cDNA克隆，命名为pα-2，发现它对电鳐乙酰胆碱受体的α亚基具有特异性。分析了两个cDNA插入片段的核苷酸序列，并确定了α链mRNA完整编码区以及部分5'和3'非翻译区的序列。给出了α链前体的完整氨基酸序列，并用于构建该多肽跨膜结构的模型。
相似文献
1
Complete mRNA coding sequence of the acetylcholine binding alpha-subunit of Torpedo marmorata acetylcholine receptor: a model for the transmembrane organization of the polypeptide chain.
Proc Natl Acad Sci U S A. 1983 Apr;80(7):2067-71. doi: 10.1073/pnas.80.7.2067.
2
Identification of a cDNA clone coding for the acetylcholine binding subunit of Torpedo marmorata acetylcholine receptor.
EMBO J. 1982;1(6):713-7. doi: 10.1002/j.1460-2075.1982.tb01235.x.
3
Isolation and characterization of a cDNA clone for the complete protein coding region of the delta subunit of the mouse acetylcholine receptor.
Proc Natl Acad Sci U S A. 1984 Dec;81(24):7970-4. doi: 10.1073/pnas.81.24.7970.
4
Molecular genetics of Torpedo marmorata acetylcholine receptor.
Adv Exp Med Biol. 1984;181:17-29. doi: 10.1007/978-1-4684-4868-9_3.
5
Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor gamma subunit.
Proc Natl Acad Sci U S A. 1983 Feb;80(4):1111-5. doi: 10.1073/pnas.80.4.1111.
6
Primary structure of alpha-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequence.
Nature. 1982 Oct 28;299(5886):793-7. doi: 10.1038/299793a0.
7
Molecular cloning of cDNA coding for the gamma subunit of Torpedo acetylcholine receptor.
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4466-70. doi: 10.1073/pnas.79.14.4466.
8
Cloning and sequence analysis of calf cDNA and human genomic DNA encoding alpha-subunit precursor of muscle acetylcholine receptor.
Nature. 1983;305(5937):818-23. doi: 10.1038/305818a0.
9
A universal oligonucleotide probe for acetylcholine receptor genes. Selection and sequencing of cDNA clones for the mouse muscle beta subunit.
J Biol Chem. 1986 Dec 15;261(35):16451-8.
10
The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor.
Nucleic Acids Res. 1982 Oct 11;10(19):5809-22. doi: 10.1093/nar/10.19.5809.
引用本文的文献
1
Wandering about allostery.
Biol Direct. 2024 Aug 8;19(1):64. doi: 10.1186/s13062-024-00502-0.
2
Highlighting membrane protein structure and function: A celebration of the Protein Data Bank.
J Biol Chem. 2021 Jan-Jun;296:100557. doi: 10.1016/j.jbc.2021.100557. Epub 2021 Mar 18.
3
Interplay between Gating and Block of Ligand-Gated Ion Channels.
Brain Sci. 2020 Dec 1;10(12):928. doi: 10.3390/brainsci10120928.
4
Structure of the Native Muscle-type Nicotinic Receptor and Inhibition by Snake Venom Toxins.
Neuron. 2020 Jun 17;106(6):952-962.e5. doi: 10.1016/j.neuron.2020.03.012. Epub 2020 Apr 9.
5
Progress in nicotinic receptor structural biology.
Neuropharmacology. 2020 Jul;171:108086. doi: 10.1016/j.neuropharm.2020.108086. Epub 2020 Apr 7.
6
Discovery of the First Neurotransmitter Receptor: The Acetylcholine Nicotinic Receptor.
Biomolecules. 2020 Apr 3;10(4):547. doi: 10.3390/biom10040547.
7
The nicotinic acetylcholine receptor: a typical 'allosteric machine'.
Philos Trans R Soc Lond B Biol Sci. 2018 Jun 19;373(1749). doi: 10.1098/rstb.2017.0174.
8
The Ascaris suum nicotinic receptor, ACR-16, as a drug target: Four novel negative allosteric modulators from virtual screening.
Int J Parasitol Drugs Drug Resist. 2016 Feb 10;6(1):60-73. doi: 10.1016/j.ijpddr.2016.02.001. eCollection 2016 Apr.
9
The concept of allosteric interaction and its consequences for the chemistry of the brain.
J Biol Chem. 2013 Sep 20;288(38):26969-26986. doi: 10.1074/jbc.X113.503375. Epub 2013 Jul 22.
10
The nicotinic acetylcholine receptor: the founding father of the pentameric ligand-gated ion channel superfamily.
J Biol Chem. 2012 Nov 23;287(48):40207-15. doi: 10.1074/jbc.R112.407668. Epub 2012 Oct 4.
本文引用的文献
1
ON THE NATURE OF ALLOSTERIC TRANSITIONS: A PLAUSIBLE MODEL.
J Mol Biol. 1965 May;12:88-118. doi: 10.1016/s0022-2836(65)80285-6.
2
Reconstitution of a functional acetylcholine receptor. Conservation of the conformational and allosteric transitions and recovery of the permeability response; role of lipids.
Eur J Biochem. 1980 Sep;110(1):35-55. doi: 10.1111/j.1432-1033.1980.tb04839.x.
3
Acetylcholine receptor: complex of homologous subunits.
Science. 1980 Jun 27;208(4451):1454-6. doi: 10.1126/science.7384786.
4
Membrane proteins: the amino acid composition of membrane-penetrating segments.
Eur J Biochem. 1981 Nov;120(2):275-8. doi: 10.1111/j.1432-1033.1981.tb05700.x.
5
6
On the hydrophobic nature of signal sequences.
Eur J Biochem. 1981 May 15;116(2):419-22. doi: 10.1111/j.1432-1033.1981.tb05351.x.
7
Reconstitution of acetylcholine receptor function using purified receptor protein.
Biochemistry. 1981 Feb 17;20(4):694-701. doi: 10.1021/bi00507a004.
8
Structure and function of an acetylcholine receptor.
Biophys J. 1982 Jan;37(1):371-83. doi: 10.1016/S0006-3495(82)84685-7.
9
Specific labeling of 3' termini with T4 DNA polymerase.
Methods Enzymol. 1980;65(1):39-43. doi: 10.1016/s0076-6879(80)65008-3.
10
Molecular cloning of cDNA coding for the gamma subunit of Torpedo acetylcholine receptor.
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4466-70. doi: 10.1073/pnas.79.14.4466.
Zotero 插件