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电鳐乙酰胆碱受体乙酰胆碱结合α亚基的完整mRNA编码序列：多肽链跨膜组织的模型

Complete mRNA coding sequence of the acetylcholine binding alpha-subunit of Torpedo marmorata acetylcholine receptor: a model for the transmembrane organization of the polypeptide chain.

作者信息

Devillers-Thiery A, Giraudat J, Bentaboulet M, Changeux J P

出版信息

Proc Natl Acad Sci U S A. 1983 Apr;80(7):2067-71. doi: 10.1073/pnas.80.7.2067.

DOI:10.1073/pnas.80.7.2067
PMID:6572962
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC393754/
Abstract

A 1,350-base-pair-long cDNA clone, named p alpha-2, was isolated by hybridization to the previously characterized clone p alpha-1 and found to be specific for the alpha-subunit of the Torpedo marmorata acetylcholine receptor. The nucleotide sequences of both cDNA inserts were analyzed and the sequence of the complete coding region and part of the 5' and 3' untranslated regions of the alpha-chain mRNA was determined. The complete amino acid sequence of the alpha-chain precursor is presented and used to develop a model for the transmembrane organization of the polypeptide.

摘要

通过与先前鉴定的克隆pα-1杂交，分离出一个长度为1350个碱基对的cDNA克隆，命名为pα-2，发现它对电鳐乙酰胆碱受体的α亚基具有特异性。分析了两个cDNA插入片段的核苷酸序列，并确定了α链mRNA完整编码区以及部分5'和3'非翻译区的序列。给出了α链前体的完整氨基酸序列，并用于构建该多肽跨膜结构的模型。

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Complete mRNA coding sequence of the acetylcholine binding alpha-subunit of Torpedo marmorata acetylcholine receptor: a model for the transmembrane organization of the polypeptide chain.电鳐乙酰胆碱受体乙酰胆碱结合α亚基的完整mRNA编码序列：多肽链跨膜组织的模型
Proc Natl Acad Sci U S A. 1983 Apr;80(7):2067-71. doi: 10.1073/pnas.80.7.2067.
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