Claudio T, Ballivet M, Patrick J, Heinemann S
Proc Natl Acad Sci U S A. 1983 Feb;80(4):1111-5. doi: 10.1073/pnas.80.4.1111.
The nucleotide sequence has been determined of a cDNA clone that codes for the 60,000-dalton gamma subunit of Torpedo californica acetylcholine receptor. The length of the cDNA clone is 2,010 base pairs. The 5' and 3' untranslated regions have respective lengths of 31 and 461 base pairs. Data suggest that the putative polyadenylylation consensus sequence A-A-T-A-A-A may not be required for polyadenylylation of the mRNA corresponding to the cDNA clone described in this study. From the DNA sequence data, the amino acid sequence of the gamma subunit was deduced. The subunit is composed of 489 amino acids giving a molecular mass of 56,600 daltons. The deduced amino acid sequence data also indicate the presence of a 17-amino acid extension or signal peptide on this subunit. From these data, structural predictions for the gamma subunit are made such as potential membrane-spanning regions, possible asparagine-linked glycosylation sites, and the assignment of regions of the protein to the extracellular, internal, and cytoplasmic domains of the lipid bilayer.
已经确定了一个编码加州电鳐乙酰胆碱受体60,000道尔顿γ亚基的cDNA克隆的核苷酸序列。该cDNA克隆的长度为2,010个碱基对。5'和3'非翻译区的长度分别为31和461个碱基对。数据表明，本研究中描述的与cDNA克隆相对应的mRNA的聚腺苷酸化可能不需要推定的聚腺苷酸化共有序列A-A-T-A-A-A。根据DNA序列数据，推导了γ亚基的氨基酸序列。该亚基由489个氨基酸组成，分子量为56,600道尔顿。推导的氨基酸序列数据还表明该亚基上存在一个17个氨基酸的延伸或信号肽。根据这些数据，对γ亚基进行了结构预测，如潜在的跨膜区域、可能的天冬酰胺连接的糖基化位点，以及将蛋白质区域分配到脂质双层的细胞外、内部和细胞质结构域。
