Plant Molecular Biology, Division of Biological Sciences, Monsanto Company, 700 Chesterfield Village Parkway, St Louis, MO 63198, USA.
EMBO J. 1987 May;6(5):1181-8. doi: 10.1002/j.1460-2075.1987.tb02352.x.
A chimeric gene encoding the alfalfa mosaic virus (AlMV) coat protein was constructed and introduced into tobacco and tomato plants using Ti plasmid-derived plant transformation vectors. The progeny of the self-fertilized transgenic plants were significantly delayed in symptom development and in some cases completely escaped infection after inoculated with AlMV. The inoculated leaves of the transgenic plants had significantly reduced numbers of lesions and accumulated substantially lower amounts of coat protein due to virus replication than the control plants. These results show that high level expression of the chimeric viral coat protein gene confers protection against AlMV, which differs from other plant viruses in morphology, genome structure, gene expression strategy and early steps in viral replication. Based on our results with AlMV and those reported earlier for tobacco mosaic virus, it appears that genetically engineered cross-protection may be a general method for preventing viral disease in plants.
构建了一个嵌合基因,该基因编码紫花苜蓿花叶病毒(AlMV)外壳蛋白,并使用 Ti 质粒衍生的植物转化载体将其导入烟草和番茄植物。自交的转基因植物的后代在症状发展方面明显延迟,并且在接种 AlMV 后在某些情况下完全逃脱感染。与对照植物相比,转基因植物的接种叶片中由于病毒复制而导致的病变数量明显减少,并且积累的外壳蛋白量也大大降低。这些结果表明,嵌合病毒外壳蛋白基因的高水平表达赋予了对 AlMV 的保护作用,这与形态、基因组结构、基因表达策略以及病毒复制的早期步骤均不同的其他植物病毒不同。基于我们在 AlMV 上的结果以及早期在烟草花叶病毒上的报道,看来基因工程交叉保护可能是预防植物病毒病的一般方法。