苜蓿花叶病毒RNA3的完整核苷酸序列
Complete nucleotide sequence of alfalfa mosaic virus RNA3.
作者信息
Barker R F, Jarvis N P, Thompson D V, Loesch-Fries L S, Hall T C
出版信息
Nucleic Acids Res. 1983 May 11;11(9):2881-91. doi: 10.1093/nar/11.9.2881.
Abstract
A full-length cDNA clone of alfalfa mosaic virus (AMV) RNA3 was prepared and sequenced. The 2,037 base sequence contains two open reading frames of 903 and 666 nucleotides that code for a 32,400 dalton protein (32.4K protein) and the 24,380 dalton coat protein, respectively. A 5'-noncoding sequence of 240 bases preceeding the 32.4K protein contains homologous regions that may have a function in its translation. The intercistronic junction is 49 bases long, the last 36 bases representing the 5'-end of the subgenomic RNA4. The remaining 179 bases comprise the 3'-terminal noncoding sequence.
摘要
制备并测序了苜蓿花叶病毒（AMV）RNA3的全长cDNA克隆。2037个碱基的序列包含两个分别为903和666个核苷酸的开放阅读框，它们分别编码一个32400道尔顿的蛋白质（32.4K蛋白质）和24380道尔顿的外壳蛋白。在32.4K蛋白质之前有一段240个碱基的5'-非编码序列，其中包含可能在其翻译过程中起作用的同源区域。顺反子间连接区长49个碱基，最后36个碱基代表亚基因组RNA4的5'-末端。其余179个碱基构成3'-末端非编码序列。
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