Ravelonandro M, Pinck M, Pinck L
Biochimie. 1984 May;66(5):395-402. doi: 10.1016/0300-9084(84)90023-3.
We report the sequence of RNA 3 from strain S of Alfalfa mosaic virus (2,055 nucleotides). This RNA codes for a 32.4 kd protein (P3) and for the 24 kd coat protein (P4). The largest part of the sequence was established using RNA sequencing methods. The completion of the sequence in the region coding for P3 was achieved with cloned cDNA synthesized after priming at internal sites of RNA 3. Comparison of the RNA sequences coding P3 and P4 proteins in strain S with those reported in the literature for strain 425 revealed a higher amino acid substitution rate (3%) for P3 than for P4 (congruent to 1%) despite a similar average base substitution of 3-4% in these regions. In P3, two out of nine amino acid changes occur in hydrophilic regions. The amino acid changes in P4 do not modify the local hydrophilicity distribution. The intercistronic region displays a low degree of base substitution (2%) when compared with the untranslated 3'-end region (3.6%) or the 5'-end leader region (8%), the average substitution rate being 3.2%.
我们报道了苜蓿花叶病毒S株系RNA 3的序列（2055个核苷酸）。该RNA编码一个32.4千道尔顿的蛋白（P3）和24千道尔顿的外壳蛋白（P4）。序列的大部分是用RNA测序方法确定的。通过在RNA 3内部位点引发后合成的克隆cDNA完成了P3编码区的序列。将S株系中编码P3和P4蛋白的RNA序列与文献中报道的425株系的序列进行比较，发现尽管这些区域的平均碱基替换率相似，为3-4%，但P3的氨基酸替换率（3%）高于P4（约为1%）。在P3中，九个氨基酸变化中有两个发生在亲水区。P4中的氨基酸变化不改变局部亲水性分布。与未翻译的3'端区域（3.6%）或5'端前导区域（8%）相比，基因间区域的碱基替换程度较低（2%），平均替换率为3.2%。
