Medical Research Council, Laboratory of Molecular Biology, University Medical School, Hills Road, Cambridge CB2 2QH, UK.
EMBO J. 1987 Jun;6(6):1765-73. doi: 10.1002/j.1460-2075.1987.tb02429.x.
In Drosophila the process of segmentation depends on the function of coordinate, gap, pair-rule and segment-polarity genes. Mutations in segment-polarity genes cause defects in the pattern of every segment. Here the cloning of sequences from a segment-polarity gene, wingless, and the in situ localization of a transcript in embryos are described. The transcript is first detected in the anterior and posterior regions of the blastoderm embryo at cellularization, and accumulates in a series of stripes in the extended germ band, one stripe per metameric unit. Each stripe is localized to the most posterior cells of each parasegment. The signal is predominantly epidermal, and transcript accumulates only transiently in the mesoderm and nervous system. This pattern of expression is discussed with respect to models of pattern formation in segmental units.
在果蝇中，体节的形成过程依赖于同源、间隙、配对规则和体节极性基因的功能。体节极性基因突变会导致每个体节模式的缺陷。这里描述了一个体节极性基因 wingless 的序列克隆及其在胚胎中的转录本的原位定位。在胚胎细胞化时，转录本首先在前、后胚层中被检测到，并在延伸的 germ band 中积累成一系列条纹，每一个体节单元一个条纹。每个条纹都定位于每个副体节的最后端细胞。信号主要是表皮的，转录本只在中胚层和神经系统中短暂积累。这种表达模式与分段单元的模式形成模型有关。
