通过染色体步移和跳查从黑腹果蝇的Ace和玫瑰色基因座以及双胸复合体中分离DNA。

Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster.

作者信息

Bender W, Spierer P, Hogness D S

出版信息

J Mol Biol. 1983 Jul 25;168(1):17-33. doi: 10.1016/s0022-2836(83)80320-9.

Abstract

A chromosomal walk is described that covers 315 X 10(3) base-pairs of DNA from the 87DE region of the third chromosome of Drosophila melanogaster. The walk includes the DNA for the rosy and Ace loci, which code for xanthine dehydrogenase and acetylcholinesterase, respectively. Several dispersed repetitive elements were encountered in the walk. In every case, their positions in the chromosome differed in different strains, and so they are all presumed to be transposable elements. Several rearrangement breakpoints have been localized within the walk, including the break for In(3R) Cbx+R1 (87E1, 2-89E1, 2). One breakpoint fusion fragment of this inversion was isolated to jump from 87E into the cluster of homeotic genes of the bithorax complex, at 89E1-4.

摘要

描述了一种染色体步移，它覆盖了果蝇第三染色体87DE区域的315×10³个碱基对的DNA。该步移包括编码黄嘌呤脱氢酶和乙酰胆碱酯酶的 rosy和Ace基因座的DNA。在步移过程中遇到了几个分散的重复元件。在每种情况下，它们在不同菌株的染色体中的位置都不同，因此推测它们都是可转座元件。几个重排断点已定位在步移范围内，包括In(3R)Cbx+R1(87E1,2-89E1,2)的断点。该倒位的一个断点融合片段被分离出来，从87E跳入双胸复合体同源异型基因簇，位于89E1-4。