Department of Medical Chemistry, University of Helsinki, Siltavuorenpenger 10,00170 Helsinki 17, Finland.
EMBO J. 1987 Sep;6(9):2825-33. doi: 10.1002/j.1460-2075.1987.tb02579.x.
Synthetic oligonucleotide probes were used to clone two loci from the chromosomal DNA of Paracoccus denitrificans that contain the genes for cytochrome c oxidase (cytochrome aa(3)). One locus seems to contain four or five genes probably forming an operon. Two of these code for the oxidase subunits II and III. Three open reading frames are found between the COII and COIII genes. The other locus codes for the subunit I. A short open reading frame is found upstream of this gene. All three subunits of the Paracoccus enzyme show remarkable homology to the corresponding subunits of the mitochondrial cytochrome oxidase. Possible protein products of the open reading frames have not yet been identified.
合成寡核苷酸探针被用于从 Paracoccus denitrificans 的染色体 DNA 中克隆出两个包含细胞色素 c 氧化酶（细胞色素 aa(3)）基因的基因座。一个基因座似乎包含四个或五个基因，可能形成一个操纵子。其中两个编码氧化酶亚基 II 和 III。在 COII 和 COIII 基因之间发现了三个开放阅读框。另一个基因座编码亚基 I。在这个基因的上游发现了一个短的开放阅读框。该酶的三个亚基与线粒体细胞色素氧化酶的相应亚基表现出显著的同源性。开放阅读框的可能蛋白质产物尚未被鉴定。
