Department of Genetics, Downing Street, Cambridge CB2 3EH, UK.
EMBO J. 1988 Apr;7(4):1107-14. doi: 10.1002/j.1460-2075.1988.tb02919.x.
We describe the structure and expression of an exceptional bithorax complex mutation, C1. This deletion mutation removes major portions of both the Ultrabithorax (Ubx) and abdominal-A (abd-A) protein coding regions, yet retains many of the functions normally executed by these genes. We show that the ends of the C1 deletion map to analogous positions within the Ubx and abd-A transcription units, and that the deletion chromosome gives rise to a set of novel fusion transcripts that may encode hybrid abd-A/Ubx proteins. These fusion products are transcribed from the abd-A promoter, but exhibit a novel spatial pattern of expression that combines aspects of the normal Ubx and abd-A distributions. This pattern provides evidence for the existence of regulatory elements located in the 3' region of the Ubx transcription unit that can act on the abd-A promoter at a distance of at least 20 kb.
我们描述了一个特殊的双胸复合体突变 C1 的结构和表达。这个缺失突变去除了 Ultrabithorax（Ubx）和 Abdominal-A（Abd-A）蛋白编码区的大部分，但保留了这些基因通常执行的许多功能。我们表明，C1 缺失的末端映射到 Ubx 和 Abd-A 转录单元中的类似位置，并且缺失染色体产生了一组可能编码杂交 Abd-A/Ubx 蛋白的新融合转录本。这些融合产物由 Abd-A 启动子转录，但表现出一种新的表达空间模式，结合了正常 Ubx 和 Abd-A 分布的各个方面。该模式为存在位于 Ubx 转录单元 3' 区域的调控元件提供了证据，这些调控元件可以在至少 20 kb 的距离处作用于 Abd-A 启动子。
