Dente L, Cesareni G, Cortese R
Nucleic Acids Res. 1983 Mar 25;11(6):1645-55. doi: 10.1093/nar/11.6.1645.
We have constructed a series of plasmids, the pEMBL family, characterized by the presence of 1) the bla gene as selectable marker, 2) a short segment coding for the alpha-peptide of beta-galactosidase and containing a multiple cloning sites polylinker, 3) the intragenic region of phage F1. pEMBL plasmids have the property of being encapsidated as single stranded DNA, upon superinfection with phage F1. These vectors have been used successfully for DNA sequencing with the dideoxy-method, and can be used for any other purpose for which M13 derivatives are used. However, the pEMBL plasmids have the advantage of being smaller than M13 vectors, and the purification of the DNA is simpler. In addition, and most importantly, long inserts have a higher stability in pEMBL plasmids than M13 vectors.
我们构建了一系列质粒，即pEMBL家族，其特征在于存在：1）作为选择标记的bla基因；2）一段编码β-半乳糖苷酶α-肽并包含多克隆位点多接头的短片段；3）噬菌体F1的基因内区域。pEMBL质粒具有在被噬菌体F1超感染时被包装成单链DNA的特性。这些载体已成功用于双脱氧法DNA测序，并且可用于任何其他使用M13衍生物的目的。然而，pEMBL质粒具有比M13载体更小的优势，并且DNA的纯化更简单。此外，最重要的是，长插入片段在pEMBL质粒中比在M13载体中具有更高的稳定性。
