MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
EMBO J. 1985 Aug;4(8):2035-43. doi: 10.1002/j.1460-2075.1985.tb03889.x.
We have used a monoclonal antibody to examine the distribution of Ultrabithorax (Ubx) proteins in Drosophila embryos and imaginal discs by immunofluorescence. Ubx proteins are nuclear and show a spatially restricted distribution in the nervous system, epidermis and mesoderm. Labelling extends from the first thoracic segment (T1) to the eighth abdominal segment (A8) in the midline cells, from T2 to A8 in the ventral nervous system and epidermis and from A1 to A8 in the somatic mesoderm. In the nervous systems and epidermis the patterns of labelling exhibit a repeat unit, the Ubx metamere, that is out of phase with the segmental repeat unit. At least in the epidermis this repeat unit appears to extend between anterior-posterior compartment boundaries and consists of a posterior compartment together with the succeeding anterior compartment. The most prominently labelled metamere in the nervous system and epidermis is that comprising the posterior region of T3 and the anterior region of A1. Within each metamere the nuclei are heterogeneously labelled. Clear heterogeneity of labelling is also seen amongst the nuclei of the T3 imaginal discs.
我们使用单克隆抗体通过免疫荧光法研究了果蝇胚胎和成虫盘中超常盘（Ubx）蛋白的分布。Ubx 蛋白位于细胞核内，并在神经系统、表皮和中胚层中表现出空间限制分布。标记物从中线细胞的第一胸节（T1）延伸到第八腹节（A8），从 T2 到腹侧神经系统和表皮的 A8，以及从 A1 到 A8 的体节中胚层。在神经系统和表皮中，标记的模式表现出重复单元，即 Ubx 体节，与节段重复单元不同步。至少在表皮中，这个重复单元似乎在前后区室边界之间延伸，由后区室和随后的前区室组成。在神经系统和表皮中标记最明显的体节是由 T3 的后区和 A1 的前区组成的体节。在每个体节中，细胞核呈异质性标记。在 T3 成虫盘的细胞核中也可以看到明显的标记异质性。
