Biology Department C016, University of California San Diego, La Jolla, CA 92093, USA.
EMBO J. 1988 Jun;7(6):1589-96. doi: 10.1002/j.1460-2075.1988.tb02984.x.
Maize streak virus (MSV), a geminivirus with a one-component genome, encodes a major coat protein RNA which accumulates in infected plants. Using a maize protoplast cell transient expression system, we have defined and studied the promoter which drives rightward transcription of the RNA encoding the coat protein. We have identified a 122 bp upstream segment that enhances promoter activity and functions as an upstream activating sequence (UAS). The UAS lies in the starting intergenic region of the viral genome and includes a region which is similar in all geminiviruses. The 122 bp UAS activates the MSV core promoter in an orientation, but not position, independent fashion. The MSV promoter UAS is interchangeable with a similar element in the cauliflower mosaic virus (CaMV) 35S RNA core promoter, that is the MSV UAS will activate the CaMV 35S core promoter and vice versa. However, the MSV promoter UAS specifically binds proteins in maize nuclear extracts which appear to differ from those bound by the functionally equivalent region of the CaMV 35S promoter.
玉米线条病毒（MSV）是一种单组分基因组的双生病毒，编码一种主要的外壳蛋白 RNA，在感染的植物中积累。我们使用玉米原生质体细胞瞬时表达系统，定义并研究了驱动 RNA 编码外壳蛋白向右转录的启动子。我们已经确定了一个 122 个碱基对的上游片段，该片段增强了启动子的活性，并作为一个上游激活序列（UAS）发挥作用。UAS 位于病毒基因组的起始内含子区，包含一个在所有双生病毒中都相似的区域。122 个碱基对的 UAS 以一种定向但不依赖于位置的方式激活 MSV 核心启动子。MSV 启动子 UAS 可与花椰菜花叶病毒（CaMV）35S RNA 核心启动子中的类似元件互换，即 MSV UAS 可激活 CaMV 35S 核心启动子，反之亦然。然而，MSV 启动子 UAS 特异性结合玉米核提取物中的蛋白质，这些蛋白质似乎与 CaMV 35S 启动子功能等效区域结合的蛋白质不同。
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