Department of Botany, University of Florida, Gainesville, FL 32611.
Proc Natl Acad Sci U S A. 1987 Feb;84(3):799-803. doi: 10.1073/pnas.84.3.799.
DNase-I-hypersensitive sites have been characterized in a plant gene, maize Adh1 (which encodes alcohol dehydrogenase 1). It has been generally recognized in animal genes that the chromatin of the 5' flanking region can be characterized by the accessibility of its DNA to the nuclease DNase I (EC 3.1.21.1), indicating which areas in the promoter are "open" to nuclear factors. The 5' region of the maize Adh1 gene contains two distinct DNase-I-hypersensitive regions, one constitutively present from position -160 to -700 and one that is anaerobically induced from position -35 to -150. The constitutive region contains three major hypersensitive sites, one of which corresponds in part to a region of potential Z-DNA. The induced hypersensitive region includes TATAA at -38 and CAAT at -100 as well as other potential regulatory sequences.
DNase-I 超敏位点已在植物基因玉米 Adh1（编码醇脱氢酶 1）中得到鉴定。人们普遍认为，动物基因的 5'侧翼区染色质可以通过其 DNA 对核酸酶 DNase I（EC 3.1.21.1）的可及性来表征，这表明启动子中的哪些区域对核因子“开放”。玉米 Adh1 基因的 5'区域包含两个不同的 DNase-I 超敏区域，一个从位置-160 到-700 持续存在，另一个从位置-35 到-150 厌氧诱导。组成型区域包含三个主要的超敏位点，其中一个部分对应于潜在 Z-DNA 的区域。诱导的超敏区域包括-38 处的 TATAA 和-100 处的 CAAT 以及其他潜在的调控序列。
Zotero 插件
