Tang J L, Liu Y N, Barber C E, Dow J M, Wootton J C, Daniels M J
Sainsbury Laboratory, John Innes Centre for Plant Science Research, Norwich, UK.
Mol Gen Genet. 1991 May;226(3):409-17. doi: 10.1007/BF00260653.
The cosmid clone pIJ3020 containing DNA from the plant pathogenic bacterium Xanthomonas campestris pathovar campestris has previously been shown to complement a non-pathogenic mutant defective in synthesis of extracellular enzymes. The DNA cloned in pIJ3020 was analysed by mutagenesis with Tn5 and Tn5lac and by nucleotide sequencing. The results indicate that this region of the genome contains a cluster of genes, mutation in any of which results in failure of the enzymes and extracellular polysaccharide to be synthesized. The designation rpf (regulation of pathogenicity factors) is proposed for these genes. The nucleotide sequence of one gene (rpfC) predicts a protein product with homology to conserved domains of both sensor and regulator proteins of prokaryotic two-component regulatory systems, which are usually involved in regulating gene expression in response to environmental stimuli.
含有来自植物致病细菌野油菜黄单胞菌野油菜致病变种DNA的黏粒克隆pIJ3020，先前已被证明可互补在细胞外酶合成方面存在缺陷的非致病突变体。通过用Tn5和Tn5lac进行诱变以及核苷酸测序，对克隆于pIJ3020中的DNA进行了分析。结果表明，基因组的这一区域包含一组基因，其中任何一个基因发生突变都会导致酶和细胞外多糖无法合成。建议将这些基因命名为rpf（致病因子调控）。一个基因（rpfC）的核苷酸序列预测出一种蛋白质产物，它与原核双组分调控系统的传感器和调节蛋白的保守结构域具有同源性，这些系统通常参与响应环境刺激来调节基因表达。
