Levine Jonathan M, Ruaux Craig G, Bergman Robert L, Coates Joan R, Steiner Jörg M, Williams David A
Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.
Am J Vet Res. 2006 Feb;67(2):283-7. doi: 10.2460/ajvr.67.2.283.
To detect matrix metalloproteinase (MMP)-9 in serum and CSF and determine relationships between MMP activity and severity of disease, duration of clinical signs, and duration of hospitalization in dogs with acute intervertebral disk disease (IVDD).
35 dogs with acute IVDD and 8 clinically normal control dogs.
CSF and serum were collected from affected and control dogs. Zymography was used to detect MMP-9.
Activity of MMP-9 in CSF was detected in 6 of 35 dogs with IVDD; activity was significantly more common in dogs with duration of signs < 24 hours. Paraplegic dogs were more likely to have MMP-9 activity in the CSF than non-paraplegic dogs. No significant difference in hospitalization time was detected in dogs with IVDD between those with and without activity of MMP-9 in the CSF. Serum MMP-9 was detected more frequently in dogs with IVDD than in control dogs.
Data were consistent with results of experimental rodent spinal cord injury studies that indicate that MMP-9 is expressed early during secondary injury.
检测急性椎间盘疾病（IVDD）犬血清和脑脊液中的基质金属蛋白酶（MMP）-9，并确定MMP活性与疾病严重程度、临床症状持续时间及住院时间之间的关系。
35只患有急性IVDD的犬和8只临床正常的对照犬。
从患病犬和对照犬采集脑脊液和血清。采用酶谱法检测MMP-9。
35只患有IVDD的犬中，6只脑脊液中检测到MMP-9活性；症状持续时间<24小时的犬中，该活性更为常见。截瘫犬脑脊液中出现MMP-9活性的可能性高于非截瘫犬。脑脊液中MMP-9有活性和无活性的IVDD犬之间，住院时间无显著差异。IVDD犬血清中检测到MMP-9的频率高于对照犬。
数据与啮齿动物实验性脊髓损伤研究结果一致，表明MMP-9在继发性损伤早期表达。
