Department of Veterinary Clinical Science, Kagoshima University, Kagoshima, Japan.
Spine (Phila Pa 1976). 2010 Jan 1;35(1):4-9. doi: 10.1097/BRS.0b013e3181b61f14.
Cerebrospinal fluid (CSF) from dogs with intervertebral disc herniation (IVDH) were analyzed using a specific antibody against cartilage oligomeric matrix protein (COMP). Immunolocalization and genetic expression of COMP were also examined in the spinal cords of mouse, rat, and dog.
To investigate the hypothesis that COMP is present in CSF of dogs with IVDH, and the clinical relevance of COMP expression in CSF as a potential biomarker of spinal cord injury.
In dog IVDH, diagnostic imaging is useful for determining the spinal cord compression, but less useful for assessment of traumatic degeneration. Aggrecan, a well-known component of cartilage matrix, is increased in CSF from cases of neural damage and inflammation of the spinal cord.
CSF from the cisterna magna (C-CSF) and the lumbar spine (L-CSF) of 19 dogs with IVDH and 5 normal dogs were analyzed using inhibition enzyme-linked immunosorbent assay (ELISA) and immunoblotting with an antibody cross-reactive with dog COMP. Samples of normal spinal cord from mouse, rat, and dog were also prepared for immunohistochemistry and in situ hybridization.
ELISA values were significantly higher for L-CSF than for C-CSF in dogs with IVDH, whereas there was no significant difference between them in normal dogs. Immunoblots of L-CSF samples revealed positive bands of approximately 500 kDa in 6 cases of IVDH (positive cases), but no signal in negative cases. ELISA values were significantly higher in the positive cases than in the negative cases. Both COMP protein and mRNA were present at high levels in the gray matter of the spinal cord in all species.
In dog IVDH, release of COMP from the spinal cord in association with injury may lead to COMP accumulation in L-CSF posterior to the site of disc extrusion, and therefore might be a predictive marker of spinal cord injury.
采用针对软骨寡聚基质蛋白（COMP）的特异性抗体分析患有椎间盘突出症（IVDH）的犬的脑脊液（CSF）。还检查了 COMP 在小鼠、大鼠和犬的脊髓中的免疫定位和遗传表达。
验证 COMP 存在于患有 IVDH 的犬的 CSF 中，并验证 CSF 中 COMP 表达作为脊髓损伤潜在生物标志物的临床相关性。
在犬 IVDH 中，诊断成像对于确定脊髓受压非常有用，但对于评估创伤性退变的作用较小。聚集蛋白聚糖，一种软骨基质的已知成分，在与脊髓神经损伤和炎症相关的 CSF 中增加。
使用抑制酶联免疫吸附试验（ELISA）和与犬 COMP 交叉反应的抗体进行免疫印迹分析，分析了 19 只患有 IVDH 的犬和 5 只正常犬的脑池（C-CSF）和腰椎（L-CSF）CSF。还制备了来自小鼠、大鼠和犬的正常脊髓样本进行免疫组织化学和原位杂交。
患有 IVDH 的犬的 L-CSF 的 ELISA 值明显高于 C-CSF，而正常犬之间没有显着差异。L-CSF 样本的免疫印迹显示在 6 例 IVDH 中（阳性病例）有约 500 kDa 的阳性带，但在阴性病例中没有信号。阳性病例的 ELISA 值明显高于阴性病例。在所有物种的脊髓灰质中均存在高水平的 COMP 蛋白和 mRNA。
在犬 IVDH 中，与损伤相关的脊髓中 COMP 的释放可能导致 COMP 在椎间盘突出部位后向 L-CSF 中积累，因此可能是脊髓损伤的预测标志物。
