Green Brad R, Bulaj Grzegorz
Cognetix, Inc., 421 Wakara Way, Suite 201, Salt Lake City, Utah 84108, USA.
Protein Pept Lett. 2006;13(1):67-70. doi: 10.2174/092986606774502162.
We tested two alternative oxidation strategies to produce conotoxins alpha-GI and micro-PIIIA. The peptides were either reversibly immobilized on a solid support and then oxidized, or the immobilized disulfide reagent (CLEAR-OX(trade mark)) was used to oxidize the peptides. Both strategies appeared more efficient at higher peptide concentrations, consistent with pseudo-dilution effects.
我们测试了两种用于生产芋螺毒素α-GI和微小-PIIIA的替代氧化策略。这些肽要么被可逆地固定在固相载体上然后进行氧化，要么使用固定化的二硫键试剂（CLEAR-OX（商标））来氧化这些肽。两种策略在较高肽浓度下似乎更有效，这与假稀释效应一致。
