Ichimura Mana, Hiratsuka Koichi, Ogura Naomi, Utsunomiya Tadahiko, Sakamaki Hiroyuki, Kondoh Toshirou, Abiko Yoshimitsu, Otake Shigeo, Yamamoto Masafumi
Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
J Oral Pathol Med. 2006 Mar;35(3):167-74. doi: 10.1111/j.1600-0714.2006.00402.x.
To understand the immunopathological features of oral lichen planus (OLP), we analyzed the expression of chemokines in the epithelial cell layers.
Epithelia from OLP or healthy gingiva were collected by laser microdissection. The chemokine and chemokine receptor expressions in the epithelia were analyzed by DNA microarray.
High levels of MIP-3alpha/LARC/CCL20 and its receptor CCR6 were expressed in the lesional epithelia. Furthermore, DC-CK1/CCL18, ELC/CCL19, SDF-1/CXCL12 and CXCR4 expressions were also increased. Immunohistologial analysis showed that high numbers of Langerhans cells (LCs) were present in the epithelia of OLP. Lesional epithelia also expressed high levels of the ligands specific for CXCR3 (e.g. MIG/CXCL9, IP-10/CXCL10 and I-TAC/CXCL11) and CCR5 (e.g. RANTES/CCL5).
Infiltration of LCs is orchestrated by CCR6. Further, LCs residing in the lesional epithelia may be a mature phenotype. Moreover, infiltration of T cells in OLP could be mediated by signaling pathways through CXCR3 and CCR5.
为了解口腔扁平苔藓（OLP）的免疫病理特征，我们分析了趋化因子在上皮细胞层中的表达。
通过激光显微切割收集OLP或健康牙龈的上皮组织。采用DNA微阵列分析上皮组织中趋化因子及其受体的表达。
病变上皮中MIP-3α/LARC/CCL20及其受体CCR6表达水平较高。此外，DC-CK1/CCL18、ELC/CCL19、SDF-1/CXCL12和CXCR4的表达也增加。免疫组织学分析显示，OLP上皮中有大量朗格汉斯细胞（LCs）。病变上皮中还高水平表达CXCR3特异性配体（如MIG/CXCL9、IP-10/CXCL10和I-TAC/CXCL11）和CCR5特异性配体（如RANTES/CCL5）。
LCs的浸润由CCR6调控。此外，病变上皮中的LCs可能是成熟表型。而且，OLP中T细胞的浸润可能由通过CXCR3和CCR5的信号通路介导。
