Yoon Kyung-Chul, De Paiva Cintia S, Qi Hong, Chen Zhuo, Farley William J, Li De-Quan, Pflugfelder Stephen C
Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA.
Invest Ophthalmol Vis Sci. 2007 Jun;48(6):2561-9. doi: 10.1167/iovs.07-0002.
To evaluate the effects of desiccating ocular surface stress on the expression of chemokines and their receptors by the corneal epithelium and conjunctiva of C57BL/6 and BALB/c mice.
Experimental dry eye was created in C57BL/6 and BALB/c mice. The concentrations of macrophage inflammatory protein 1alpha (MIP-1alpha), MIP-1beta, monokine induced by interferon (MIG)-gamma, and interferon-gamma-inducible protein (IP)-10 in the corneal epithelia and conjunctiva were measured by a multiplex immunobead assay. Expression of MIP-1alpha; MIP-1beta; regulated on activation, normal T-cell expressed and secreted (RANTES), MIG, IP-10; monocyte chemoattractant protein (MCP)-3; eotaxin-1; CCR5; CXCR3; and CCR3 in the cornea and conjunctiva were evaluated by real-time PCR and immunostaining.
Desiccating stress significantly increased concentrations of MIP-1alpha, MIP-1beta, IP-10, and MIG proteins in the corneal epithelium and conjunctiva of C57BL/6 mice. Furthermore, it increased levels of MIP-1alpha, MIP-1beta, and CCR5 transcripts in the cornea and conjunctiva and RANTES, MIG, IP-10, and CXCR3 transcripts in the conjunctiva of C57BL/6 mice. In contrast, levels of MCP-3, eotaxin-1, and CCR3 transcripts increased in both tissues of BALB/c mice. In situ immunodetection of chemokines and their receptors was similar to their pattern of gene expression.
Specific patterns of Th-1 and -2 chemokines and their receptors are induced in the mouse ocular surface by desiccating stress in a strain-related fashion. Desiccating stress potently stimulates the expression of Th-1 cell-attracting chemokines and chemokine receptors on the ocular surface of C57BL/6 mice.
评估干燥性眼表应激对C57BL/6和BALB/c小鼠角膜上皮和结膜中趋化因子及其受体表达的影响。
在C57BL/6和BALB/c小鼠中制造实验性干眼。通过多重免疫磁珠分析法测量角膜上皮和结膜中巨噬细胞炎性蛋白1α（MIP-1α）、MIP-1β、干扰素诱导的单核因子（MIG）-γ和干扰素-γ诱导蛋白（IP）-10的浓度。通过实时聚合酶链反应和免疫染色评估角膜和结膜中MIP-1α、MIP-1β、活化调节正常T细胞表达和分泌因子（RANTES）、MIG、IP-10、单核细胞趋化蛋白（MCP）-3、嗜酸性粒细胞趋化因子-1、C-C趋化因子受体5（CCR5）、C-X-C趋化因子受体3（CXCR3）和CCR3的表达。
干燥应激显著增加了C57BL/6小鼠角膜上皮和结膜中MIP-1α、MIP-1β、IP-10和MIG蛋白的浓度。此外，它增加了C57BL/6小鼠角膜和结膜中MIP-1α、MIP-1β和CCR5转录本的水平以及结膜中RANTES、MIG、IP-10和CXCR3转录本的水平。相比之下，BALB/c小鼠的两个组织中MCP-3、嗜酸性粒细胞趋化因子-1和CCR3转录本的水平增加。趋化因子及其受体的原位免疫检测与其基因表达模式相似。
干燥应激以品系相关的方式在小鼠眼表诱导Th-1和Th-2趋化因子及其受体的特定模式。干燥应激有力地刺激了C57BL/6小鼠眼表吸引Th-1细胞的趋化因子和趋化因子受体的表达。
