Lu Jia, Moochhala Shabbir, Moore Xiao-Lei, Ng Kian Chye, Tan Mui Hong, Lee Lionel Kim Hock, He Beiping, Wong Meng Cheong, Ling Eng-Ang
Defence Medical and Environmental Research Institute, DSO National Laboratories, 27 Medical Drive, #09-01, Singapore 117510, Singapore.
Neurosci Lett. 2006 May 1;398(1-2):12-7. doi: 10.1016/j.neulet.2005.12.053. Epub 2006 Feb 7.
This study aims to investigate the therapeutic potential of adult bone marrow stromal cells (BMSCs). Exposed to a cocktail of induction medium, some BMSCs could differentiate into cell types with phenotypes of neural lineages in vitro. These cells expressed neural markers nestin, GFAP, 68-kDa neurofilament and beta-tubulin III as detected by immunohistochemistry and RT-PCR. Fluorescence-labeled cells were injected intravenously at 72 h after traumatic brain injury. Transplanted cells survived and migrated to the ipsilateral cerebral cortex at different time points after injection. They were immunopositive for neuronal marker MAP-2, oligodendrocyte marker CNPase, astrocytic maker GFAP or microglial marker OX-42 in vivo. In rats receiving BMSC transplants, there were significant improvements in motor and neurological functions when compared with the control groups. Hence, the therapeutic potential of BMSCs for traumatic brain injury is further amplified.
本研究旨在探讨成人骨髓基质细胞（BMSCs）的治疗潜力。暴露于诱导培养基混合物中后，一些BMSCs在体外可分化为具有神经谱系表型的细胞类型。通过免疫组织化学和逆转录-聚合酶链反应检测发现，这些细胞表达神经标志物巢蛋白、胶质纤维酸性蛋白、68 kDa神经丝和β-微管蛋白III。在创伤性脑损伤后72小时静脉注射荧光标记的细胞。注射后不同时间点，移植细胞存活并迁移至同侧大脑皮层。在体内，它们对神经元标志物微管相关蛋白2（MAP-2）、少突胶质细胞标志物2',3'-环核苷酸3'-磷酸二酯酶（CNPase）、星形胶质细胞标志物胶质纤维酸性蛋白（GFAP）或小胶质细胞标志物OX-42呈免疫阳性。与对照组相比，接受BMSC移植的大鼠运动和神经功能有显著改善。因此，BMSCs对创伤性脑损伤的治疗潜力得到进一步增强。
